Force measurements between bacteria and poly(ethylene glycol)-coated surfaces

Anneta Razatos, Yea Ling Ong, Fabienne Boulay, Donald L. Elbert, Jeffrey A. Hubbell, Mukul M. Sharma, George Georgiou

Research output: Contribution to journalArticle

102 Citations (Scopus)

Abstract

The atomic force microscope (AFM) was used to directly measure the forces of interaction between E. coli D21 bacteria and hydrophilic glass or hydrophobic N-octadecyltrichlorosilane (OTS)-treated glass substrates coated with the block copolymers, poly(ethylene glycol) (PEG)-lysine dendron or Pluronic F127 surfactant, respectively. Short-range repulsive interactions between bacterial cells and substrates coated with the block copolymers were detected by the AFM over distances of separation comparable to the extended length of the PEG polymer chains. In contrast, glass and OTS-treated glass devoid of PEG-lysine dendron or Pluronic F127 exerted long-range attractive forces on E. coli D21 bacteria. Thus, polymeric brush layers appear to not only block the long-range attractive forces of interaction between bacteria and substrates but also introduce repulsive steric effects.

Original languageEnglish
Pages (from-to)9155-9158
Number of pages4
JournalLangmuir
Volume16
Issue number24
DOIs
StatePublished - Nov 28 2000
Externally publishedYes

Fingerprint

UCON 50-HB-5100
Force measurement
bacteria
Polyethylene glycols
glycols
Bacteria
ethylene
Poloxamer
Glass
Escherichia coli
glass
lysine
Lysine
Block copolymers
block copolymers
Microscopes
Substrates
microscopes
Brushes
Surface-Active Agents

ASJC Scopus subject areas

  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry

Cite this

Razatos, A., Ong, Y. L., Boulay, F., Elbert, D. L., Hubbell, J. A., Sharma, M. M., & Georgiou, G. (2000). Force measurements between bacteria and poly(ethylene glycol)-coated surfaces. Langmuir, 16(24), 9155-9158. https://doi.org/10.1021/la000818y

Force measurements between bacteria and poly(ethylene glycol)-coated surfaces. / Razatos, Anneta; Ong, Yea Ling; Boulay, Fabienne; Elbert, Donald L.; Hubbell, Jeffrey A.; Sharma, Mukul M.; Georgiou, George.

In: Langmuir, Vol. 16, No. 24, 28.11.2000, p. 9155-9158.

Research output: Contribution to journalArticle

Razatos, A, Ong, YL, Boulay, F, Elbert, DL, Hubbell, JA, Sharma, MM & Georgiou, G 2000, 'Force measurements between bacteria and poly(ethylene glycol)-coated surfaces', Langmuir, vol. 16, no. 24, pp. 9155-9158. https://doi.org/10.1021/la000818y
Razatos A, Ong YL, Boulay F, Elbert DL, Hubbell JA, Sharma MM et al. Force measurements between bacteria and poly(ethylene glycol)-coated surfaces. Langmuir. 2000 Nov 28;16(24):9155-9158. https://doi.org/10.1021/la000818y
Razatos, Anneta ; Ong, Yea Ling ; Boulay, Fabienne ; Elbert, Donald L. ; Hubbell, Jeffrey A. ; Sharma, Mukul M. ; Georgiou, George. / Force measurements between bacteria and poly(ethylene glycol)-coated surfaces. In: Langmuir. 2000 ; Vol. 16, No. 24. pp. 9155-9158.
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