Force and compliance measurements on living cells using atomic force microscopy (AFM)

Ewa P. Wojcikiewicz, Xiaohui Zhang, Vincent T. Moy

Research output: Contribution to journalArticle

110 Scopus citations

Abstract

We describe the use of atomic force microscopy (AFM) in studies of cell adhesion and cell compliance. Our studies use the interaction between leukocyte function associated antigen-1 (LFA-1)/intercellular adhesion molecule-1 (ICAM-1) as a model system. The forces required to unbind a single LFA-1/ICAM-1 bond were measured at different loading rates. This data was used to determine the dynamic strength of the LFA-1/ICAM-1 complex and characterize the activation potential that this complex overcomes during its breakage. Force measurements acquired at the multiple- bond level provided insight about the mechanism of cell adhesion. In addition, the AFM was used as a microindenter to determine the mechanical properties of cells. The applications of these methods are described using data from a previous study.

Original languageEnglish (US)
Pages (from-to)1-9
Number of pages9
JournalBiological Procedures Online
Volume6
Issue number1
DOIs
StatePublished - Jan 15 2004

Keywords

  • Atomic force
  • Cell adhesion
  • Leukocytes
  • Microscopy

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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