Abstract
We describe the use of atomic force microscopy (AFM) in studies of cell adhesion and cell compliance. Our studies use the interaction between leukocyte function associated antigen-1 (LFA-1)/intercellular adhesion molecule-1 (ICAM-1) as a model system. The forces required to unbind a single LFA-1/ICAM-1 bond were measured at different loading rates. This data was used to determine the dynamic strength of the LFA-1/ICAM-1 complex and characterize the activation potential that this complex overcomes during its breakage. Force measurements acquired at the multiple- bond level provided insight about the mechanism of cell adhesion. In addition, the AFM was used as a microindenter to determine the mechanical properties of cells. The applications of these methods are described using data from a previous study.
| Original language | English |
|---|---|
| Pages (from-to) | 1-9 |
| Number of pages | 9 |
| Journal | Biological Procedures Online |
| Volume | 6 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 15 2004 |
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Keywords
- Atomic force
- Cell adhesion
- Leukocytes
- Microscopy
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Force and compliance measurements on living cells using atomic force microscopy (AFM). / Wojcikiewicz, Ewa P.; Zhang, Xiaohui; Moy, Vincent T.
In: Biological Procedures Online, Vol. 6, No. 1, 15.01.2004, p. 1-9.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Force and compliance measurements on living cells using atomic force microscopy (AFM)
AU - Wojcikiewicz, Ewa P.
AU - Zhang, Xiaohui
AU - Moy, Vincent T.
PY - 2004/1/15
Y1 - 2004/1/15
N2 - We describe the use of atomic force microscopy (AFM) in studies of cell adhesion and cell compliance. Our studies use the interaction between leukocyte function associated antigen-1 (LFA-1)/intercellular adhesion molecule-1 (ICAM-1) as a model system. The forces required to unbind a single LFA-1/ICAM-1 bond were measured at different loading rates. This data was used to determine the dynamic strength of the LFA-1/ICAM-1 complex and characterize the activation potential that this complex overcomes during its breakage. Force measurements acquired at the multiple- bond level provided insight about the mechanism of cell adhesion. In addition, the AFM was used as a microindenter to determine the mechanical properties of cells. The applications of these methods are described using data from a previous study.
AB - We describe the use of atomic force microscopy (AFM) in studies of cell adhesion and cell compliance. Our studies use the interaction between leukocyte function associated antigen-1 (LFA-1)/intercellular adhesion molecule-1 (ICAM-1) as a model system. The forces required to unbind a single LFA-1/ICAM-1 bond were measured at different loading rates. This data was used to determine the dynamic strength of the LFA-1/ICAM-1 complex and characterize the activation potential that this complex overcomes during its breakage. Force measurements acquired at the multiple- bond level provided insight about the mechanism of cell adhesion. In addition, the AFM was used as a microindenter to determine the mechanical properties of cells. The applications of these methods are described using data from a previous study.
KW - Atomic force
KW - Cell adhesion
KW - Leukocytes
KW - Microscopy
UR - http://www.scopus.com/inward/record.url?scp=2342608476&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=2342608476&partnerID=8YFLogxK
U2 - 10.1251/bpo67
DO - 10.1251/bpo67
M3 - Article
AN - SCOPUS:2342608476
VL - 6
SP - 1
EP - 9
JO - Biological Procedures Online
JF - Biological Procedures Online
SN - 1480-9222
IS - 1
ER -