Focusing on RISC assembly in mammalian cells

Junmei Hong, Na Wei, Alistair Chalk, Jue Wang, Yutong Song, Fan Yi, Ren Ping Qiao, Erik L L Sonnhammer, Claes R Wahlestedt, Zicai Liang, Quan Du

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

RISC (RNA-induced silencing complex) is a central protein complex in RNAi, into which a siRNA strand is assembled to become effective in gene silencing. By using an in vitro RNAi reaction based on Drosophila embryo extract, an asymmetric model was recently proposed for RISC assembly of siRNA strands, suggesting that the strand that is more loosely paired at its 5′ end is selectively assembled into RISC and results in target gene silencing. However, in the present study, we were unable to establish such a correlation in cell-based RNAi assays, as well as in large-scale RNAi data analyses. This suggests that the thermodynamic stability of siRNA is not a major determinant of gene silencing in mammalian cells. Further studies on fork siRNAs showed that mismatch at the 5′ end of the siRNA sense strand decreased RISC assembly of the antisense strand, but surprisingly did not increase RISC assembly of the sense strand. More interestingly, measurements of melting temperature showed that the terminal stability of fork siRNAs correlated with the positions of the mismatches, but not gene silencing efficacy. In summary, our data demonstrate that there is no definite correlation between siRNA stability and gene silencing in mammalian cells, which suggests that instead of thermodynamic stability, other features of the siRNA duplex contribute to RISC assembly in RNAi.

Original languageEnglish
Pages (from-to)703-708
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume368
Issue number3
DOIs
StatePublished - Apr 11 2008
Externally publishedYes

Fingerprint

RNA-Induced Silencing Complex
Small Interfering RNA
Gene Silencing
RNA Interference
Cells
Genes
Thermodynamics
Thermodynamic stability
Freezing
Drosophila
Melting point
Assays
Embryonic Structures
Temperature

Keywords

  • RISC assembly
  • Silencing activity
  • siRNA
  • Thermodynamic stability

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Hong, J., Wei, N., Chalk, A., Wang, J., Song, Y., Yi, F., ... Du, Q. (2008). Focusing on RISC assembly in mammalian cells. Biochemical and Biophysical Research Communications, 368(3), 703-708. https://doi.org/10.1016/j.bbrc.2008.01.116

Focusing on RISC assembly in mammalian cells. / Hong, Junmei; Wei, Na; Chalk, Alistair; Wang, Jue; Song, Yutong; Yi, Fan; Qiao, Ren Ping; Sonnhammer, Erik L L; Wahlestedt, Claes R; Liang, Zicai; Du, Quan.

In: Biochemical and Biophysical Research Communications, Vol. 368, No. 3, 11.04.2008, p. 703-708.

Research output: Contribution to journalArticle

Hong, J, Wei, N, Chalk, A, Wang, J, Song, Y, Yi, F, Qiao, RP, Sonnhammer, ELL, Wahlestedt, CR, Liang, Z & Du, Q 2008, 'Focusing on RISC assembly in mammalian cells', Biochemical and Biophysical Research Communications, vol. 368, no. 3, pp. 703-708. https://doi.org/10.1016/j.bbrc.2008.01.116
Hong, Junmei ; Wei, Na ; Chalk, Alistair ; Wang, Jue ; Song, Yutong ; Yi, Fan ; Qiao, Ren Ping ; Sonnhammer, Erik L L ; Wahlestedt, Claes R ; Liang, Zicai ; Du, Quan. / Focusing on RISC assembly in mammalian cells. In: Biochemical and Biophysical Research Communications. 2008 ; Vol. 368, No. 3. pp. 703-708.
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