Fluorouracil (5-FU) and cytarabine (ara-C) inhibition of corneal epithelial cell and conjunctival fibroblast proliferation

K. S. Mallick, A. S. Hajek, Richard K Parrish

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Abstract

We evaluated the antiproliferative properties of fluorouracil (5-FU) and cytarabine (ara-C) in a tissue culture model of rabbit corneal epithelial cells and conjunctival fibroblasts. Cells were treated with fluorouracil or cytarabine for five days. Corneal epithelial cell proliferation was inhibited to 50% of control proliferation by 0.6 mg/L of fluorouracil or 30 μg/L of cytarabine. Proliferation of conjunctival fibroblasts was inhibited to 50% of control by 0.5 mg/L of fluorouracil or 50 μg/L of cytarabine. The antiproliferative effect of 10 mg/L of fluorouracil was not blocked by thymidine. The inhibition of proliferation produced by 100 or 1,000 μg/L of cytarabine was blocked by 2'-deoxycytidine at concentrations of 10-5M and 10-4M, respectively. The antiproliferative effects of fluorouracil and cytarabine appear similar for both cell types. Blockage of cytarabine-related ocular toxic effects may be possible with 2'-deoxycytidine.

Original languageEnglish
Pages (from-to)1398-1402
Number of pages5
JournalArchives of Ophthalmology
Volume103
Issue number9
StatePublished - Jan 1 1985

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Cytarabine
Fluorouracil
Fibroblasts
Epithelial Cells
Deoxycytidine
Poisons
Thymidine
Cell Proliferation
Rabbits

ASJC Scopus subject areas

  • Ophthalmology

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Fluorouracil (5-FU) and cytarabine (ara-C) inhibition of corneal epithelial cell and conjunctival fibroblast proliferation. / Mallick, K. S.; Hajek, A. S.; Parrish, Richard K.

In: Archives of Ophthalmology, Vol. 103, No. 9, 01.01.1985, p. 1398-1402.

Research output: Contribution to journalArticle

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