Fluorouracil (5-FU) and Cytarabine (Ara-C) Inhibition of Corneal Epithelial Cell and Conjunctival Fibroblast Proliferation

Kim S. Mallick, Anthony S. Hajek, Richard K. Parrish

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

We evaluated the antiproliferative properties of fluorouracil (5-FU) and cytarabine (ara-C) in a tissue culture model of rabbit corneal epithelial cells and conjunctival fibroblasts. Cells were treated with fluorouracil or cytarabine for five days. Corneal epithelial cell proliferation was inhibited to 50% of control proliferation by 0.6 mg/L of fluorouracil or 30 μg/L of cytarabine. Proliferation of conjunctival fibroblasts was inhibited to 50% of control by 0.5 mg/L of fluorouracil or 50 μg/L of cytarabine. The antiproliferative effect of 10 mg/L of fluorouracil was not blocked by thymidine. The inhibition of proliferation produced by 100 or 1,000 μg/L of cytarabine was blocked by 2'-deoxycytidine at concentrations of 10-5M and 10-4M, respectively. The antiproliferative effects of fluorouracil and cytarabine appear similar for both cell types. Blockage of cytarabine-related ocular toxic effects may be possible with 2'-deoxycytidine.

Original languageEnglish (US)
Pages (from-to)1398-1402
Number of pages5
JournalArchives of ophthalmology
Volume103
Issue number9
DOIs
StatePublished - Sep 1985

ASJC Scopus subject areas

  • Ophthalmology

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