Fluorimetric determination of aldose reductased in small tissue samples

Sven E. Brolin, Per Olof Berggren, Peter Naeser

Research output: Contribution to journalArticlepeer-review

Abstract

The assay is based on the consumption of NADPH catalyzed by the enzyme, with glucose as substrate, and measurement of the native NADPH fluorescence. This modification yields 10-fold higher sensitivity than the usual procedure based on absorbance measurements at 340 nm. The method is applied to tissue samples weighing 0.1-2 mg.

Original languageEnglish (US)
Pages (from-to)363-367
Number of pages5
JournalAnalytica Chimica Acta
Volume206
Issue numberC
DOIs
StatePublished - 1988

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

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