Fluorimetric determination of aldose reductased in small tissue samples

Sven E. Brolin, Per Olof Berggren, Peter Naeser

Research output: Contribution to journalArticle

Abstract

The assay is based on the consumption of NADPH catalyzed by the enzyme, with glucose as substrate, and measurement of the native NADPH fluorescence. This modification yields 10-fold higher sensitivity than the usual procedure based on absorbance measurements at 340 nm. The method is applied to tissue samples weighing 0.1-2 mg.

Original languageEnglish (US)
Pages (from-to)363-367
Number of pages5
JournalAnalytica Chimica Acta
Volume206
Issue numberC
DOIs
StatePublished - 1988

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

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