Fluorimetric determination of aldose reductased in small tissue samples

Sven E. Brolin, Per Olof Berggren, Peter Naeser

Research output: Contribution to journalArticle

Abstract

The assay is based on the consumption of NADPH catalyzed by the enzyme, with glucose as substrate, and measurement of the native NADPH fluorescence. This modification yields 10-fold higher sensitivity than the usual procedure based on absorbance measurements at 340 nm. The method is applied to tissue samples weighing 0.1-2 mg.

Original languageEnglish
Pages (from-to)363-367
Number of pages5
JournalAnalytica Chimica Acta
Volume206
Issue numberC
DOIs
StatePublished - Jan 1 1988
Externally publishedYes

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NADP
absorbance
glucose
fluorescence
assay
Tissue
enzyme
fold
substrate
Weighing
Assays
Fluorescence
Glucose
Substrates
Enzymes
consumption
method
tissue

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry

Cite this

Fluorimetric determination of aldose reductased in small tissue samples. / Brolin, Sven E.; Berggren, Per Olof; Naeser, Peter.

In: Analytica Chimica Acta, Vol. 206, No. C, 01.01.1988, p. 363-367.

Research output: Contribution to journalArticle

Brolin, Sven E. ; Berggren, Per Olof ; Naeser, Peter. / Fluorimetric determination of aldose reductased in small tissue samples. In: Analytica Chimica Acta. 1988 ; Vol. 206, No. C. pp. 363-367.
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