Fluorimetric determination of aldose reductase in small tissue samples

S. E. Brolin; P. O. Berggren; P. Naeser

doi:10.1016/S0003-2670(00)80857-0

Fluorimetric determination of aldose reductase in small tissue samples

S. E. Brolin, P. O. Berggren, P. Naeser

Surgery

Research output: Contribution to journal › Article

4 Scopus citations

Abstract

The assay is based on the consumption of NADPH catalyzed by the enzyme, with glucose as substrate, and measurement of the native NADPH fluorescence. This modification yields 10-fold higher sensitivity than the usual procedure based on absorbance measurements at 340 nm. The method is applied to tissue samples weighing 0.1-2 mg.

Original language	English (US)
Pages (from-to)	357-361
Number of pages	5
Journal	Analytica Chimica Acta
Volume	206
Issue number	1-2
DOIs	https://doi.org/10.1016/S0003-2670(00)80857-0
State	Published - Jan 1 1988

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Environmental Chemistry
Spectroscopy

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Brolin, S. E., Berggren, P. O., & Naeser, P. (1988). Fluorimetric determination of aldose reductase in small tissue samples. Analytica Chimica Acta, 206(1-2), 357-361. https://doi.org/10.1016/S0003-2670(00)80857-0

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