Fluorimetric determination of aldose reductase in small tissue samples

S. E. Brolin, P. O. Berggren, P. Naeser

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


The assay is based on the consumption of NADPH catalyzed by the enzyme, with glucose as substrate, and measurement of the native NADPH fluorescence. This modification yields 10-fold higher sensitivity than the usual procedure based on absorbance measurements at 340 nm. The method is applied to tissue samples weighing 0.1-2 mg.

Original languageEnglish (US)
Pages (from-to)357-361
Number of pages5
JournalAnalytica Chimica Acta
Issue number1-2
StatePublished - Jan 1 1988

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy


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