Abstract
The assay is based on the consumption of NADPH catalyzed by the enzyme, with glucose as substrate, and measurement of the native NADPH fluorescence. This modification yields 10-fold higher sensitivity than the usual procedure based on absorbance measurements at 340 nm. The method is applied to tissue samples weighing 0.1-2 mg.
Original language | English (US) |
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Pages (from-to) | 357-361 |
Number of pages | 5 |
Journal | Analytica Chimica Acta |
Volume | 206 |
Issue number | 1-2 |
DOIs | |
State | Published - Jan 1 1988 |
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Environmental Chemistry
- Spectroscopy