Fluorimetric determination of aldose reductase in small tissue samples

S. E. Brolin, P. O. Berggren, P. Naeser

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

The assay is based on the consumption of NADPH catalyzed by the enzyme, with glucose as substrate, and measurement of the native NADPH fluorescence. This modification yields 10-fold higher sensitivity than the usual procedure based on absorbance measurements at 340 nm. The method is applied to tissue samples weighing 0.1-2 mg.

Original languageEnglish (US)
Pages (from-to)357-361
Number of pages5
JournalAnalytica Chimica Acta
Volume206
Issue number1-2
DOIs
StatePublished - Jan 1 1988

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

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