Fluorimetric determination of aldose reductase in small tissue samples

S. E. Brolin; P. O. Berggren; P. Naeser

doi:10.1016/S0003-2670(00)80857-0

Fluorimetric determination of aldose reductase in small tissue samples

S. E. Brolin, P. O. Berggren, P. Naeser

Surgery

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Abstract

The assay is based on the consumption of NADPH catalyzed by the enzyme, with glucose as substrate, and measurement of the native NADPH fluorescence. This modification yields 10-fold higher sensitivity than the usual procedure based on absorbance measurements at 340 nm. The method is applied to tissue samples weighing 0.1-2 mg.

Original language	English (US)
Pages (from-to)	357-361
Number of pages	5
Journal	Analytica Chimica Acta
Volume	206
Issue number	1-2
DOIs	https://doi.org/10.1016/S0003-2670(00)80857-0
State	Published - Jan 1 1988

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Environmental Chemistry
Spectroscopy

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10.1016/S0003-2670(00)80857-0

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title = "Fluorimetric determination of aldose reductase in small tissue samples",

abstract = "The assay is based on the consumption of NADPH catalyzed by the enzyme, with glucose as substrate, and measurement of the native NADPH fluorescence. This modification yields 10-fold higher sensitivity than the usual procedure based on absorbance measurements at 340 nm. The method is applied to tissue samples weighing 0.1-2 mg.",

author = "Brolin, {S. E.} and Berggren, {P. O.} and P. Naeser",

year = "1988",

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T1 - Fluorimetric determination of aldose reductase in small tissue samples

AU - Brolin, S. E.

AU - Berggren, P. O.

AU - Naeser, P.

PY - 1988/1/1

Y1 - 1988/1/1

N2 - The assay is based on the consumption of NADPH catalyzed by the enzyme, with glucose as substrate, and measurement of the native NADPH fluorescence. This modification yields 10-fold higher sensitivity than the usual procedure based on absorbance measurements at 340 nm. The method is applied to tissue samples weighing 0.1-2 mg.

AB - The assay is based on the consumption of NADPH catalyzed by the enzyme, with glucose as substrate, and measurement of the native NADPH fluorescence. This modification yields 10-fold higher sensitivity than the usual procedure based on absorbance measurements at 340 nm. The method is applied to tissue samples weighing 0.1-2 mg.

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U2 - 10.1016/S0003-2670(00)80857-0

DO - 10.1016/S0003-2670(00)80857-0

M3 - Article

AN - SCOPUS:0023938217

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SP - 357

EP - 361

JO - Analytica Chimica Acta

JF - Analytica Chimica Acta

SN - 0003-2670

IS - 1-2

ER -

Fluorimetric determination of aldose reductase in small tissue samples

Abstract

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