Fluorescent ion-selective optode membranes incorporated onto a centrifugal microfluidics platform

Ibrahim H.A. Badr, R. Daniel Johnson, Marc J. Madou, Leonidas G. Bachas

Research output: Contribution to journalArticlepeer-review

76 Scopus citations


The development of an integrated analysis system for small ions based on ion-selective optodes and centrifugal microfluidics is reported. The performance of this system was evaluated through five-point calibration plots for two types of optode membranes, one being cation-selective and the other anion-selective, which were incorporated into a microfluidics platform on which fluid motion is induced via angular rotation. Additionally, the application of the microfluidic platform to ion analysis is studied via a two-point calibration protocol used to quantify an unknown sample. Calibrant solutions are delivered from reservoirs fabricated onto the platform to a measuring area that contains the optode membrane, with a change in membrane fluorescence being monitored. This work demonstrates the first instance of a microfluidic-based analysis system with detection based on ion-selective optode membranes monitored with fluorescence transduction. Furthermore, in addition to employing a standard excitation source where a fiber-optic probe is coupled to a tungsten-halogen lamp, laser diodes such as those employed in portable CD/DVD players were studied as excitation sources to enhance the observed fluorescence signals.

Original languageEnglish (US)
Pages (from-to)5569-5575
Number of pages7
JournalAnalytical Chemistry
Issue number21
StatePublished - Nov 1 2002
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry


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