Fluorescent in situ hybridization on archival G-banded slides

W. El-Rifai; S. Knuutila

doi:10.1159/000134367

Fluorescent in situ hybridization on archival G-banded slides

W. El-Rifai, S. Knuutila

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

We report a simple procedure that allows the application of chromosomal in situ suppression hybridization to standard G-banded and Entellan-mounted archival preparations that had been stored for 4-8 y. The procedure includes removing the cover slips in xylene, refixation in buffered formaldehyde-acetone mixture, and washing in 2 − SSC, Tween 20.

Original language	English (US)
Pages (from-to)	322-324
Number of pages	3
Journal	Cytogenetic and Genome Research
Volume	73
Issue number	4
DOIs	https://doi.org/10.1159/000134367
State	Published - Jan 1 1996
Externally published	Yes

ASJC Scopus subject areas

Molecular Biology
Genetics
Genetics(clinical)

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10.1159/000134367

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title = "Fluorescent in situ hybridization on archival G-banded slides",

abstract = "We report a simple procedure that allows the application of chromosomal in situ suppression hybridization to standard G-banded and Entellan-mounted archival preparations that had been stored for 4-8 y. The procedure includes removing the cover slips in xylene, refixation in buffered formaldehyde-acetone mixture, and washing in 2 − SSC, Tween 20.",

author = "W. El-Rifai and S. Knuutila",

year = "1996",

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doi = "10.1159/000134367",

language = "English (US)",

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AU - Knuutila, S.

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N2 - We report a simple procedure that allows the application of chromosomal in situ suppression hybridization to standard G-banded and Entellan-mounted archival preparations that had been stored for 4-8 y. The procedure includes removing the cover slips in xylene, refixation in buffered formaldehyde-acetone mixture, and washing in 2 − SSC, Tween 20.

AB - We report a simple procedure that allows the application of chromosomal in situ suppression hybridization to standard G-banded and Entellan-mounted archival preparations that had been stored for 4-8 y. The procedure includes removing the cover slips in xylene, refixation in buffered formaldehyde-acetone mixture, and washing in 2 − SSC, Tween 20.

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JO - Cytogenetic and Genome Research

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SN - 1424-8581

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Fluorescent in situ hybridization on archival G-banded slides

Abstract

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