Fluorescent in situ hybridization on archival G-banded slides

Wael El-Rifai, S. Knuutila

Research output: Contribution to journal › Article

5 Citations (Scopus)

Abstract

We report a simple procedure that allows the application of chromosomal in situ suppression hybridization to standard G-banded and Entellan-mounted archival preparations that had been stored for 4-8 y. The procedure includes removing the cover slips in xylene, refixation in buffered formaldehyde-acetone mixture, and washing in 2 − SSC, Tween 20.

Original languageEnglish (US)
Pages (from-to)322-324
Number of pages3
JournalCytogenetic and Genome Research
Volume73
Issue number4
DOIs
StatePublished - Jan 1 1996
Externally publishedYes

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Fluorescence In Situ Hybridization
Xylenes
Polysorbates
Acetone
Formaldehyde
In Situ Hybridization
entellan

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

Cite this

Fluorescent in situ hybridization on archival G-banded slides. / El-Rifai, Wael; Knuutila, S.

In: Cytogenetic and Genome Research, Vol. 73, No. 4, 01.01.1996, p. 322-324.

Research output: Contribution to journal › Article

El-Rifai, W & Knuutila, S 1996, 'Fluorescent in situ hybridization on archival G-banded slides', Cytogenetic and Genome Research, vol. 73, no. 4, pp. 322-324. https://doi.org/10.1159/000134367
El-Rifai W, Knuutila S. Fluorescent in situ hybridization on archival G-banded slides. Cytogenetic and Genome Research. 1996 Jan 1;73(4):322-324. https://doi.org/10.1159/000134367
El-Rifai, Wael ; Knuutila, S. / Fluorescent in situ hybridization on archival G-banded slides. In: Cytogenetic and Genome Research. 1996 ; Vol. 73, No. 4. pp. 322-324.
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