Fluorescent in situ hybridization on archival G-banded slides

W. El-Rifai, S. Knuutila

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

We report a simple procedure that allows the application of chromosomal in situ suppression hybridization to standard G-banded and Entellan-mounted archival preparations that had been stored for 4-8 y. The procedure includes removing the cover slips in xylene, refixation in buffered formaldehyde-acetone mixture, and washing in 2 − SSC, Tween 20.

Original languageEnglish (US)
Pages (from-to)322-324
Number of pages3
JournalCytogenetic and Genome Research
Volume73
Issue number4
DOIs
StatePublished - Jan 1 1996
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

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