Abstract
Luminescent whole-cell biosensing systems have been developed for a variety of analytes of environmental, clinical, and biological interest. These analytical tools allow for sensitive, rapid, simple, and inexpensive quantitative detection of target analytes. Furthermore, they can be designed to be nonspecific, semispecific, or highly specific/selective. A notable feature of such sensing systems employing living cells is that they provide information on the analyte bioavailability and activity. These characteristics, along with their suitability to miniaturization, make cell-based sensors ideal for field applications. However, a major limitation to on-site use is their "shelf-life." To address this problem, various methods for preservation of sensing cells have been reported, including freeze-drying, immobilization in different types of matrices, and formation of spores. Among these, the use of spores emerged as a promising strategy for long-term storage of whole-cell sensing systems at room temperature as well as in extreme environmental conditions.
| Original language | English |
|---|---|
| Title of host publication | Advances in Biochemical Engineering/Biotechnology |
| Pages | 57-75 |
| Number of pages | 19 |
| Volume | 117 |
| DOIs | |
| State | Published - Aug 19 2010 |
| Externally published | Yes |
Publication series
| Name | Advances in Biochemical Engineering/Biotechnology |
|---|---|
| Volume | 117 |
| ISSN (Print) | 07246145 |
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Keywords
- Bioluminescence
- Cell preservation
- Fluorescence
- Spores
- Whole-cell biosensing systems
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology
Cite this
Fluorescent and bioluminescent cell-based sensors : Strategies for their preservation. / Date, Amol; Pasini, Patrizia; Daunert, Sylvia.
Advances in Biochemical Engineering/Biotechnology. Vol. 117 2010. p. 57-75 (Advances in Biochemical Engineering/Biotechnology; Vol. 117).Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Fluorescent and bioluminescent cell-based sensors
T2 - Strategies for their preservation
AU - Date, Amol
AU - Pasini, Patrizia
AU - Daunert, Sylvia
PY - 2010/8/19
Y1 - 2010/8/19
N2 - Luminescent whole-cell biosensing systems have been developed for a variety of analytes of environmental, clinical, and biological interest. These analytical tools allow for sensitive, rapid, simple, and inexpensive quantitative detection of target analytes. Furthermore, they can be designed to be nonspecific, semispecific, or highly specific/selective. A notable feature of such sensing systems employing living cells is that they provide information on the analyte bioavailability and activity. These characteristics, along with their suitability to miniaturization, make cell-based sensors ideal for field applications. However, a major limitation to on-site use is their "shelf-life." To address this problem, various methods for preservation of sensing cells have been reported, including freeze-drying, immobilization in different types of matrices, and formation of spores. Among these, the use of spores emerged as a promising strategy for long-term storage of whole-cell sensing systems at room temperature as well as in extreme environmental conditions.
AB - Luminescent whole-cell biosensing systems have been developed for a variety of analytes of environmental, clinical, and biological interest. These analytical tools allow for sensitive, rapid, simple, and inexpensive quantitative detection of target analytes. Furthermore, they can be designed to be nonspecific, semispecific, or highly specific/selective. A notable feature of such sensing systems employing living cells is that they provide information on the analyte bioavailability and activity. These characteristics, along with their suitability to miniaturization, make cell-based sensors ideal for field applications. However, a major limitation to on-site use is their "shelf-life." To address this problem, various methods for preservation of sensing cells have been reported, including freeze-drying, immobilization in different types of matrices, and formation of spores. Among these, the use of spores emerged as a promising strategy for long-term storage of whole-cell sensing systems at room temperature as well as in extreme environmental conditions.
KW - Bioluminescence
KW - Cell preservation
KW - Fluorescence
KW - Spores
KW - Whole-cell biosensing systems
UR - http://www.scopus.com/inward/record.url?scp=77955568982&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77955568982&partnerID=8YFLogxK
U2 - 10.1007/10_2009_22
DO - 10.1007/10_2009_22
M3 - Chapter
C2 - 20091290
AN - SCOPUS:77955568982
SN - 9783642008641
VL - 117
T3 - Advances in Biochemical Engineering/Biotechnology
SP - 57
EP - 75
BT - Advances in Biochemical Engineering/Biotechnology
ER -