Fluorescent and bioluminescent cell-based sensors: Strategies for their preservation

Amol Date, Patrizia Pasini, Sylvia Daunert

Research output: Chapter in Book/Report/Conference proceedingChapter

27 Scopus citations

Abstract

Luminescent whole-cell biosensing systems have been developed for a variety of analytes of environmental, clinical, and biological interest. These analytical tools allow for sensitive, rapid, simple, and inexpensive quantitative detection of target analytes. Furthermore, they can be designed to be nonspecific, semispecific, or highly specific/selective. A notable feature of such sensing systems employing living cells is that they provide information on the analyte bioavailability and activity. These characteristics, along with their suitability to miniaturization, make cell-based sensors ideal for field applications. However, a major limitation to on-site use is their "shelf-life." To address this problem, various methods for preservation of sensing cells have been reported, including freeze-drying, immobilization in different types of matrices, and formation of spores. Among these, the use of spores emerged as a promising strategy for long-term storage of whole-cell sensing systems at room temperature as well as in extreme environmental conditions.

Original languageEnglish (US)
Title of host publicationAdvances in Biochemical Engineering/Biotechnology
EditorsT. Scheper
Pages57-75
Number of pages19
DOIs
StatePublished - 2010

Publication series

NameAdvances in Biochemical Engineering/Biotechnology
Volume117
ISSN (Print)0724-6145

Keywords

  • Bioluminescence
  • Cell preservation
  • Fluorescence
  • Spores
  • Whole-cell biosensing systems

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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    Date, A., Pasini, P., & Daunert, S. (2010). Fluorescent and bioluminescent cell-based sensors: Strategies for their preservation. In T. Scheper (Ed.), Advances in Biochemical Engineering/Biotechnology (pp. 57-75). (Advances in Biochemical Engineering/Biotechnology; Vol. 117). https://doi.org/10.1007/10_2009_22