TY - CHAP
T1 - Fluorescent and bioluminescent cell-based sensors
T2 - Strategies for their preservation
AU - Date, Amol
AU - Pasini, Patrizia
AU - Daunert, Sylvia
N1 - Funding Information:
This work was partly supported by the National Science Foundation, Grants CHE-0416553 and CHE-0718844, the National Institute of Environmental Health Sciences, Grant P42ES07380, and the United States–Israel Binational Agricultural Research and Development Fund, Grant US-3864-06. In addition, SD would like to acknowledge support from the Gill Endowment of the College of Arts & Sciences for a Gill Professorship.
PY - 2010
Y1 - 2010
N2 - Luminescent whole-cell biosensing systems have been developed for a variety of analytes of environmental, clinical, and biological interest. These analytical tools allow for sensitive, rapid, simple, and inexpensive quantitative detection of target analytes. Furthermore, they can be designed to be nonspecific, semispecific, or highly specific/selective. A notable feature of such sensing systems employing living cells is that they provide information on the analyte bioavailability and activity. These characteristics, along with their suitability to miniaturization, make cell-based sensors ideal for field applications. However, a major limitation to on-site use is their "shelf-life." To address this problem, various methods for preservation of sensing cells have been reported, including freeze-drying, immobilization in different types of matrices, and formation of spores. Among these, the use of spores emerged as a promising strategy for long-term storage of whole-cell sensing systems at room temperature as well as in extreme environmental conditions.
AB - Luminescent whole-cell biosensing systems have been developed for a variety of analytes of environmental, clinical, and biological interest. These analytical tools allow for sensitive, rapid, simple, and inexpensive quantitative detection of target analytes. Furthermore, they can be designed to be nonspecific, semispecific, or highly specific/selective. A notable feature of such sensing systems employing living cells is that they provide information on the analyte bioavailability and activity. These characteristics, along with their suitability to miniaturization, make cell-based sensors ideal for field applications. However, a major limitation to on-site use is their "shelf-life." To address this problem, various methods for preservation of sensing cells have been reported, including freeze-drying, immobilization in different types of matrices, and formation of spores. Among these, the use of spores emerged as a promising strategy for long-term storage of whole-cell sensing systems at room temperature as well as in extreme environmental conditions.
KW - Bioluminescence
KW - Cell preservation
KW - Fluorescence
KW - Spores
KW - Whole-cell biosensing systems
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U2 - 10.1007/10_2009_22
DO - 10.1007/10_2009_22
M3 - Chapter
C2 - 20091290
AN - SCOPUS:77955568982
SN - 9783642008641
T3 - Advances in Biochemical Engineering/Biotechnology
SP - 57
EP - 75
BT - Advances in Biochemical Engineering/Biotechnology
A2 - Scheper, T.
ER -