Abstract
The fluorescence properties of chlorophyll a mixed with phytol (in a 1:100 molar ratio) were studied in Langmuir-Blodgett films. Fluorescence excitation and fluorescence emission spectra of the chlorophyll a-phytol system in Langmuir-Blodgett films, prepared from an argon-saturated subphase, have maxima at 444 nm and 684 nm respectively. Two lifetime components describe the state of chlorophyll a in Langmuir-Blodgett films: τ1 = 5.18 ns (33%) and τ2 = 1.83 ns (67%); these are assigned to single molecules of chlorophyll a and to hydrated dimers or oligomers of chlorophyll a respectively. For films prepared from an oxygen-saturated subphase, the maxima of the fluorescence excitation and fluorescence emission spectra of the chlorophyll a-phytol system are shifted to 423 nm and 678 nm respectively. The lifetime components in the time-resolved fluorescence decay curve of the film are τ1 = 3.81 ns (54%) and τ2 = 1.56 ns (46%). The observed changes in the fluorescence parameters of the chlorophyll a-phytol Langmuir-Blodgett films are discussed in view of the chemical effect of molecular oxygen on the mixed chlorophyll a-phytol monolayer at the air-water interface.
| Original language | English |
|---|---|
| Pages (from-to) | 373-381 |
| Number of pages | 9 |
| Journal | Journal of Photochemistry and Photobiology, A: Chemistry |
| Volume | 57 |
| Issue number | 1-3 |
| DOIs | |
| State | Published - Apr 10 1991 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Bioengineering
- Physical and Theoretical Chemistry
Cite this
Fluorescence studies of Langmuir-Blodgett films of chlorophyll a mixed with phytol. / Zelent, Bogumil; Munger, Gaétan; Helluy, Anouk; Leblanc, Roger.
In: Journal of Photochemistry and Photobiology, A: Chemistry, Vol. 57, No. 1-3, 10.04.1991, p. 373-381.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Fluorescence studies of Langmuir-Blodgett films of chlorophyll a mixed with phytol
AU - Zelent, Bogumil
AU - Munger, Gaétan
AU - Helluy, Anouk
AU - Leblanc, Roger
PY - 1991/4/10
Y1 - 1991/4/10
N2 - The fluorescence properties of chlorophyll a mixed with phytol (in a 1:100 molar ratio) were studied in Langmuir-Blodgett films. Fluorescence excitation and fluorescence emission spectra of the chlorophyll a-phytol system in Langmuir-Blodgett films, prepared from an argon-saturated subphase, have maxima at 444 nm and 684 nm respectively. Two lifetime components describe the state of chlorophyll a in Langmuir-Blodgett films: τ1 = 5.18 ns (33%) and τ2 = 1.83 ns (67%); these are assigned to single molecules of chlorophyll a and to hydrated dimers or oligomers of chlorophyll a respectively. For films prepared from an oxygen-saturated subphase, the maxima of the fluorescence excitation and fluorescence emission spectra of the chlorophyll a-phytol system are shifted to 423 nm and 678 nm respectively. The lifetime components in the time-resolved fluorescence decay curve of the film are τ1 = 3.81 ns (54%) and τ2 = 1.56 ns (46%). The observed changes in the fluorescence parameters of the chlorophyll a-phytol Langmuir-Blodgett films are discussed in view of the chemical effect of molecular oxygen on the mixed chlorophyll a-phytol monolayer at the air-water interface.
AB - The fluorescence properties of chlorophyll a mixed with phytol (in a 1:100 molar ratio) were studied in Langmuir-Blodgett films. Fluorescence excitation and fluorescence emission spectra of the chlorophyll a-phytol system in Langmuir-Blodgett films, prepared from an argon-saturated subphase, have maxima at 444 nm and 684 nm respectively. Two lifetime components describe the state of chlorophyll a in Langmuir-Blodgett films: τ1 = 5.18 ns (33%) and τ2 = 1.83 ns (67%); these are assigned to single molecules of chlorophyll a and to hydrated dimers or oligomers of chlorophyll a respectively. For films prepared from an oxygen-saturated subphase, the maxima of the fluorescence excitation and fluorescence emission spectra of the chlorophyll a-phytol system are shifted to 423 nm and 678 nm respectively. The lifetime components in the time-resolved fluorescence decay curve of the film are τ1 = 3.81 ns (54%) and τ2 = 1.56 ns (46%). The observed changes in the fluorescence parameters of the chlorophyll a-phytol Langmuir-Blodgett films are discussed in view of the chemical effect of molecular oxygen on the mixed chlorophyll a-phytol monolayer at the air-water interface.
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U2 - 10.1016/1010-6030(91)85031-B
DO - 10.1016/1010-6030(91)85031-B
M3 - Article
VL - 57
SP - 373
EP - 381
JO - Journal of Photochemistry and Photobiology A: Chemistry
JF - Journal of Photochemistry and Photobiology A: Chemistry
SN - 1010-6030
IS - 1-3
ER -