The shape changes observed in the fluorescence spectra of chlorophyll a dissolved in polar solvents when the solute concentration is increased from 10 -6 to 10 -4 mol dm -3 are shown to be due to reabsorption of the emitted light by the solute. Furthermore a reported anomaly of the concentration dependence of the fluorescence of chlorophyll a in diethyl ether below about 10 -10 mol dm -3 is shown to be the consequence of the presence of impurities in the solvent used.
ASJC Scopus subject areas
- Analytical Chemistry
- Atomic and Molecular Physics, and Optics