Fluorescence in situ hybridization using an old world monkey y chromosome-specific probe combined with immunofluorescence staining on rhesus monkey tissues

Xiujin Xia, Terri Rasmussen, Xavier Alvarez, Takahiro Taguchi, Marilyn Li, Vincent F. La Russa

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

To date, there is no commercially available Y chromosome probe that can be used for fluorescence in situ hybridization (FISH) for the male rhesus monkey. We have recently generated a probe for FISH with high specificity to the short arm of the rhesus monkey Y chromosome. In this study, we further describe a method that keeps the integrity of tissue-specific antigenic structures for immunofluorescence staining subsequent to FISH on paraffin-embedded rhesus monkey tissues. We have examined this technique in combination with an epithelial cell-specific marker, cytokeratin 8/18 (CK8/18), on various tissues, including jejunum, liver, kidney, and pancreas. CK8/18 and Y chromosome signals were distinctly seen simultaneously on epithelial cells from the same tissue section from male but not female monkeys. These studies indicate that our FISH immunofluorescence technique can be reliably used to identify and phenotype male cells in paraffin-embedded rhesus monkey tissues.

Original languageEnglish (US)
Pages (from-to)1115-1121
Number of pages7
JournalJournal of Histochemistry and Cytochemistry
Volume55
Issue number11
DOIs
StatePublished - Nov 1 2007

    Fingerprint

Keywords

  • Fluorescence in situ hybridization
  • Rhesus monkey
  • Y chromosome immunofluorescence

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this