Fluorescence-based flow-injection determination of biotin and biotinylated compounds

Truis Smith-Palmer, Minas S. Barbarakis, Tadeausz Cynkowski, Leonidas G. Bachas

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

A homogeneous fluorophore-linked binding assay for biotin and biotin-containing compounds has been used in a merging-zone flow-injection system. Analyses were first conducted by taking advantage of the enhancement in fluorescence intensity of a conjugate of avidin-fluorescein in the presence of biotin or biotin derivatives. Detection limits for biotin as low as 2 × 10-9 M could be achieved. A greater enhancement of fluorescence was found for a streptavidin-fluorescein conjugate. Calibration curves obtained by using the streptavidin conjugate are shown for the determination of various biotinylated compounds in the flow-injection system and in a batch mode.

Original languageEnglish (US)
Pages (from-to)287-292
Number of pages6
JournalAnalytica Chimica Acta
Volume279
Issue number2
DOIs
StatePublished - Jul 15 1993
Externally publishedYes

Keywords

  • Avidin-FITC
  • Biotin Streptavidin-FITC
  • Flow injection

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

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