Fluorescence-based flow-injection determination of biotin and biotinylated compounds

Truis Smith-Palmer; Minas S. Barbarakis; Tadeausz Cynkowski; Leonidas G. Bachas

doi:10.1016/0003-2670(93)80328-I

Fluorescence-based flow-injection determination of biotin and biotinylated compounds

Truis Smith-Palmer, Minas S. Barbarakis, Tadeausz Cynkowski, Leonidas G. Bachas

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

A homogeneous fluorophore-linked binding assay for biotin and biotin-containing compounds has been used in a merging-zone flow-injection system. Analyses were first conducted by taking advantage of the enhancement in fluorescence intensity of a conjugate of avidin-fluorescein in the presence of biotin or biotin derivatives. Detection limits for biotin as low as 2 × 10^-9 M could be achieved. A greater enhancement of fluorescence was found for a streptavidin-fluorescein conjugate. Calibration curves obtained by using the streptavidin conjugate are shown for the determination of various biotinylated compounds in the flow-injection system and in a batch mode.

Original language	English (US)
Pages (from-to)	287-292
Number of pages	6
Journal	Analytica Chimica Acta
Volume	279
Issue number	2
DOIs	https://doi.org/10.1016/0003-2670(93)80328-I
State	Published - Jul 15 1993
Externally published	Yes

Keywords

Avidin-FITC
Biotin Streptavidin-FITC
Flow injection

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Environmental Chemistry
Spectroscopy

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10.1016/0003-2670(93)80328-I

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title = "Fluorescence-based flow-injection determination of biotin and biotinylated compounds",

abstract = "A homogeneous fluorophore-linked binding assay for biotin and biotin-containing compounds has been used in a merging-zone flow-injection system. Analyses were first conducted by taking advantage of the enhancement in fluorescence intensity of a conjugate of avidin-fluorescein in the presence of biotin or biotin derivatives. Detection limits for biotin as low as 2 × 10-9 M could be achieved. A greater enhancement of fluorescence was found for a streptavidin-fluorescein conjugate. Calibration curves obtained by using the streptavidin conjugate are shown for the determination of various biotinylated compounds in the flow-injection system and in a batch mode.",

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note = "Funding Information: This work was supportedb y a grant from the National Instituteso f Health (Grant GM 40510) and from NSERC (TSP).",

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T1 - Fluorescence-based flow-injection determination of biotin and biotinylated compounds

AU - Smith-Palmer, Truis

AU - Barbarakis, Minas S.

AU - Cynkowski, Tadeausz

AU - Bachas, Leonidas G.

N1 - Funding Information: This work was supportedb y a grant from the National Instituteso f Health (Grant GM 40510) and from NSERC (TSP).

PY - 1993/7/15

Y1 - 1993/7/15

N2 - A homogeneous fluorophore-linked binding assay for biotin and biotin-containing compounds has been used in a merging-zone flow-injection system. Analyses were first conducted by taking advantage of the enhancement in fluorescence intensity of a conjugate of avidin-fluorescein in the presence of biotin or biotin derivatives. Detection limits for biotin as low as 2 × 10-9 M could be achieved. A greater enhancement of fluorescence was found for a streptavidin-fluorescein conjugate. Calibration curves obtained by using the streptavidin conjugate are shown for the determination of various biotinylated compounds in the flow-injection system and in a batch mode.

AB - A homogeneous fluorophore-linked binding assay for biotin and biotin-containing compounds has been used in a merging-zone flow-injection system. Analyses were first conducted by taking advantage of the enhancement in fluorescence intensity of a conjugate of avidin-fluorescein in the presence of biotin or biotin derivatives. Detection limits for biotin as low as 2 × 10-9 M could be achieved. A greater enhancement of fluorescence was found for a streptavidin-fluorescein conjugate. Calibration curves obtained by using the streptavidin conjugate are shown for the determination of various biotinylated compounds in the flow-injection system and in a batch mode.

KW - Avidin-FITC

KW - Biotin Streptavidin-FITC

KW - Flow injection

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Fluorescence-based flow-injection determination of biotin and biotinylated compounds

Abstract

Keywords

ASJC Scopus subject areas

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