Abstract
A homogeneous fluorophore-linked binding assay for biotin and biotin-containing compounds has been used in a merging-zone flow-injection system. Analyses were first conducted by taking advantage of the enhancement in fluorescence intensity of a conjugate of avidin-fluorescein in the presence of biotin or biotin derivatives. Detection limits for biotin as low as 2 × 10-9 M could be achieved. A greater enhancement of fluorescence was found for a streptavidin-fluorescein conjugate. Calibration curves obtained by using the streptavidin conjugate are shown for the determination of various biotinylated compounds in the flow-injection system and in a batch mode.
Original language | English (US) |
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Pages (from-to) | 287-292 |
Number of pages | 6 |
Journal | Analytica Chimica Acta |
Volume | 279 |
Issue number | 2 |
DOIs | |
State | Published - Jul 15 1993 |
Externally published | Yes |
Keywords
- Avidin-FITC
- Biotin Streptavidin-FITC
- Flow injection
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Environmental Chemistry
- Spectroscopy