Fluorescence anisotropy of cyanobacterial phycobilisomes oriented in polyvinyl alcohol (PVA) films

D. Frąckowiak, A. Dudkowiak, B. Zelent, R. M. Leblanc

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Polarized absorption (at 296 and 85 K), fluorescence, and photoacoustic (at 296 and 85 K) spectra of antenna complexes-phycobilisomes isolated from cyanobacteria Tolypothrix tenuis and Oscillatoria and embedded in isotropic and anisotropic polyvinyl alcohol films-were measured. From the sets of polarized components of emission, the anisotropy of fluorescence for the pools of differently oriented molecules was calculated. On the basis of polarized photoacoustic and emission spectra, the competition between the process of thermal deactivation of excitation and excitation energy transfer in a chain of excitation donor and acceptor chromophores of phycobilisomes is discussed.

Original languageEnglish (US)
Pages (from-to)225-234
Number of pages10
JournalJournal of Fluorescence
Volume1
Issue number4
DOIs
StatePublished - Dec 1991

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Keywords

  • Cyanobacteria
  • excitation energy transfer
  • photoacoustic spectra
  • phycobilisomes
  • polarized fluorescence
  • polyvinyl alcohol (PVA) films

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry

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