Fluorescence and phosphorescence of tryptophan in peptides of different length and sequence

Ksenija Radotić, Thor Bernt Melø, Roger Leblanc, Yaser A. Yousef, K. Razi Naqvi

Research output: Contribution to journalArticle

Abstract

To interpret accurately protein fluorescence and phosphorescence, it is essential to achieve a better understanding of the luminescence properties of tryptophan (Trp, or W) in peptides. In published literature data on luminescence of peptides of varied length are scarce. This article describes studies of fluorescence and phosphorescence properties of the eight Trp-containing synthetic peptides: WAK, AWK, SWA, KYLWE, AVSWK, WVSWAK, WAKLAWE, and AVSWAKLARE. The aim was to investigate which factors influence the fluorescence yield and phosphorescence-spectra and lifetimes. Absorption spectra, room temperature fluorescence emission and corresponding excitation spectra and time-resolved phosphorescence spectra (77 K) have been recorded; the dependence of the fluorescence quantum yield on the specific peptide and its variation with the wavelength of excitation has been studied. The changes in fluorescence yield and shape of phosphorescence spectra are explained in terms of internal electron and proton transfer. The structured phosphorescence spectrum originates from proton transfer occurring upon excitation of Trp, while electron transfer gives rise to a non-structured luminescence spectrum. There is also electron transfer from higher vibronic S1 states. In the peptides there is higher probability of electron transfer than in Trp alone. The obtained data are interpreted in light of the peptides' sequence, length and conformation.

Original languageEnglish (US)
Pages (from-to)120-128
Number of pages9
JournalJournal of Photochemistry and Photobiology B: Biology
Volume157
DOIs
StatePublished - Apr 1 2016

Fingerprint

tryptophan
phosphorescence
Tryptophan
peptides
Fluorescence
fluorescence
Peptides
electron transfer
Luminescence
Electrons
luminescence
Protons
excitation
protons
proteins
absorption spectra
life (durability)
Temperature
room temperature
wavelengths

Keywords

  • Luminescence
  • Peptides
  • Phosphorescence
  • Steady state fluorescence
  • Time resolved fluorescence
  • Tryptophan

ASJC Scopus subject areas

  • Radiation
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Biophysics

Cite this

Fluorescence and phosphorescence of tryptophan in peptides of different length and sequence. / Radotić, Ksenija; Melø, Thor Bernt; Leblanc, Roger; Yousef, Yaser A.; Naqvi, K. Razi.

In: Journal of Photochemistry and Photobiology B: Biology, Vol. 157, 01.04.2016, p. 120-128.

Research output: Contribution to journalArticle

Radotić, Ksenija ; Melø, Thor Bernt ; Leblanc, Roger ; Yousef, Yaser A. ; Naqvi, K. Razi. / Fluorescence and phosphorescence of tryptophan in peptides of different length and sequence. In: Journal of Photochemistry and Photobiology B: Biology. 2016 ; Vol. 157. pp. 120-128.
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