Fluid supplementation accelerates epithelial repair during chemical colitis

Juan F. Burgueño, Jessica K. Lang, Ana M. Santander, Irina Fernández, Ester Fernández, Julia Zaias, Maria T Abreu

Research output: Contribution to journalArticle

Abstract

The dextran sulfate sodium (DSS) model of colitis is a common animal model of inflammatory bowel disease that causes pain and distress. In this study, we aimed to determine whether fluid supplementation can be used as a welfare-based intervention to minimize animal suffering. C57Bl/6 females undergoing acute colitis by administration of 3% DSS in drinking water were supplemented with 1 mL intraperitoneal injections of NaCl and compared to non-supplemented control mice. Mouse behavior and locomotive activity were assessed on days 5-6 after DSS initiation by means of tail suspension, novel object recognition and open field activity tests. Mice were euthanized after either the acute (day 7) or the recovery phase (day 12) of colitis and inflammation, epithelial proliferation, and differentiation were assessed by means of histology, immunohistochemistry, quantitative PCR, and western blot. We found that fluid-supplemented mice had reduced signs of colitis with no alterations in behavior or locomotive activity. Furthermore, we observed an accelerated epithelial repair response after fluid hydration during the acute phase of colitis, characterized by increased crypt proliferation, activation of ERK1/2, and modulation of TGF-β1 expression. Consistent with these findings, fluid-supplemented mice had increased numbers of goblet cells, upregulated expression of differentiation markers for absorptive enterocytes, and reduced inflammation during the recovery phase. Our results show that fluid hydration does not reduce stress in DSS-treated mice but alters colitis evolution by reducing clinical signs and accelerating epithelial repair. These results argue against the routine use of fluid supplementation in DSS-treated mice.

Original languageEnglish (US)
Article numbere0215387
JournalPloS one
Volume14
Issue number4
DOIs
StatePublished - Apr 1 2019

Fingerprint

colitis
Colitis
Dextran Sulfate
sodium sulfate
Repair
dextran
Fluids
mice
Locomotives
Hydration
Animals
inflammation
Recovery
Histology
Inflammation
Hindlimb Suspension
Differentiation Antigens
Object recognition
Goblet Cells
goblet cells

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Burgueño, J. F., Lang, J. K., Santander, A. M., Fernández, I., Fernández, E., Zaias, J., & Abreu, M. T. (2019). Fluid supplementation accelerates epithelial repair during chemical colitis. PloS one, 14(4), [e0215387]. https://doi.org/10.1371/journal.pone.0215387

Fluid supplementation accelerates epithelial repair during chemical colitis. / Burgueño, Juan F.; Lang, Jessica K.; Santander, Ana M.; Fernández, Irina; Fernández, Ester; Zaias, Julia; Abreu, Maria T.

In: PloS one, Vol. 14, No. 4, e0215387, 01.04.2019.

Research output: Contribution to journalArticle

Burgueño, JF, Lang, JK, Santander, AM, Fernández, I, Fernández, E, Zaias, J & Abreu, MT 2019, 'Fluid supplementation accelerates epithelial repair during chemical colitis', PloS one, vol. 14, no. 4, e0215387. https://doi.org/10.1371/journal.pone.0215387
Burgueño JF, Lang JK, Santander AM, Fernández I, Fernández E, Zaias J et al. Fluid supplementation accelerates epithelial repair during chemical colitis. PloS one. 2019 Apr 1;14(4). e0215387. https://doi.org/10.1371/journal.pone.0215387
Burgueño, Juan F. ; Lang, Jessica K. ; Santander, Ana M. ; Fernández, Irina ; Fernández, Ester ; Zaias, Julia ; Abreu, Maria T. / Fluid supplementation accelerates epithelial repair during chemical colitis. In: PloS one. 2019 ; Vol. 14, No. 4.
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