Fluid flow regulates stromal cell organization and CCL21 expression in a tissue-engineered lymph node microenvironment

Alice Tomei, Stefanie Siegert, Mirjam R. Britschgi, Sanjiv A. Luther, Melody A. Swartz

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

In the paracortex of the lymph node (LN), T zone fibroblastic reticular cells (TRCs) orchestrate an immune response by guiding lymphocyte migration both physically, by creating three-dimensional (3D) cell networks, and chemically, by secreting the chemokines CCL19 and CCL21 that direct interactions between CCR7-expressing cells, including mature dendritic cells and naive T cells. TRCs also enwrap matrix-based conduits that transport fluid from the subcapsular sinus to high endothelial venules, and fluid flow through the draining LN rapidly increases upon tissue injury or inflammation. To determine whether fluid flow affects TRC organization or function within a 3D network, we regenerated the 3D LN T zone stromal network by culturing murine TRC clones within a macroporous polyurethane scaffold containing type I collagen and Matrigel and applying slow interstitial flow (1-23 μm/min). We show that the 3D environment and slow interstitial flow are important regulators of TRC morphology, organization, and CCL21 secretion. Without flow, CCL21 expression could not be detected. Furthermore, when flow through the LN was blocked in mice in vivo, CCL21 gene expression was down-regulated within 2 h. These results highlight the importance of lymph flow as a homeostatic regulator of constitutive TRC activity and introduce the concept that increased lymph flow may act as an early inflammatory cue to enhance CCL21 expression by TRCs, thereby ensuring efficient immune cell trafficking, lymph sampling, and immune response induction.

Original languageEnglish
Pages (from-to)4273-4283
Number of pages11
JournalJournal of Immunology
Volume183
Issue number7
DOIs
StatePublished - Oct 1 2009
Externally publishedYes

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Stromal Cells
Lymph
Lymph Nodes
Chemokine CCL19
Chemokine CCL21
Polyurethanes
Venules
Collagen Type I
Dendritic Cells
Cues
Clone Cells
Lymphocytes
Inflammation
T-Lymphocytes
Gene Expression
Wounds and Injuries

ASJC Scopus subject areas

  • Immunology
  • Medicine(all)

Cite this

Fluid flow regulates stromal cell organization and CCL21 expression in a tissue-engineered lymph node microenvironment. / Tomei, Alice; Siegert, Stefanie; Britschgi, Mirjam R.; Luther, Sanjiv A.; Swartz, Melody A.

In: Journal of Immunology, Vol. 183, No. 7, 01.10.2009, p. 4273-4283.

Research output: Contribution to journalArticle

Tomei, Alice ; Siegert, Stefanie ; Britschgi, Mirjam R. ; Luther, Sanjiv A. ; Swartz, Melody A. / Fluid flow regulates stromal cell organization and CCL21 expression in a tissue-engineered lymph node microenvironment. In: Journal of Immunology. 2009 ; Vol. 183, No. 7. pp. 4273-4283.
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