flow cytometry versus urinary cytology in the evaluation of patients with bladder cancer

W. M. Murphy, L. D. Emerson, R. W. Chandler, S. M. Moinuddin, M. S. Soloway

Research output: Contribution to journalArticle

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Abstract

We compared the roles of urinary cytology and flow cytometry in the evaluation of patients with bladder cancer in clinical practice situations at a large general hospital. Specimens included 105 bladder washings from patients being followed for urothelial carcinomas and 28 control washings from individuals undergoing cystoscopy for other reasons. Flow cytometry and cytology were performed on aliquots of the same specimen in all bladder cancer samples. When carcinoma was present at the time of specimen collection it was detected by positive cytology in 75 per cent and deoxyribonucleic acid aneuploidy in 78 per cent of the cases. Combination of flow cytometry and urinary cytology increased the diagnostic yield to 95 per cent. Flow cytometry was slightly more sensitive than urinary cytology for detection of abnormalities in specimens from noninvasive and untreated tumors but the only statistically significant difference between the 2 procedures occurred among specimens from treated invasive cancers in which flow cytometry was a less sensitive method than cytology. Abnormal deoxyribonucleic acid ploidy was documented in a few specimens from noncancer-bearing patients having diseases associated with high urothelial cell turnover rates but the concomitant urinary cytology was negative for neoplasia. When used in conjunction with urinary cytology, flow cytometry was a valuable procedure in the followup of patients with bladder cancer. The diagnostic yield with this combination was such that flow cytometry and cytology may be used to reduce the frequency of cystoscopy and biopsy during clinical management in selected situations.

Original languageEnglish
Pages (from-to)815-819
Number of pages5
JournalJournal of Urology
Volume136
Issue number4
StatePublished - Dec 3 1986
Externally publishedYes

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Urinary Bladder Neoplasms
Cell Biology
Flow Cytometry
Cystoscopy
Carcinoma
Specimen Handling
Neoplasms
Ploidies
DNA
Aneuploidy
General Hospitals
Urinary Bladder
Biopsy

ASJC Scopus subject areas

  • Urology

Cite this

Murphy, W. M., Emerson, L. D., Chandler, R. W., Moinuddin, S. M., & Soloway, M. S. (1986). flow cytometry versus urinary cytology in the evaluation of patients with bladder cancer. Journal of Urology, 136(4), 815-819.

flow cytometry versus urinary cytology in the evaluation of patients with bladder cancer. / Murphy, W. M.; Emerson, L. D.; Chandler, R. W.; Moinuddin, S. M.; Soloway, M. S.

In: Journal of Urology, Vol. 136, No. 4, 03.12.1986, p. 815-819.

Research output: Contribution to journalArticle

Murphy, WM, Emerson, LD, Chandler, RW, Moinuddin, SM & Soloway, MS 1986, 'flow cytometry versus urinary cytology in the evaluation of patients with bladder cancer', Journal of Urology, vol. 136, no. 4, pp. 815-819.
Murphy WM, Emerson LD, Chandler RW, Moinuddin SM, Soloway MS. flow cytometry versus urinary cytology in the evaluation of patients with bladder cancer. Journal of Urology. 1986 Dec 3;136(4):815-819.
Murphy, W. M. ; Emerson, L. D. ; Chandler, R. W. ; Moinuddin, S. M. ; Soloway, M. S. / flow cytometry versus urinary cytology in the evaluation of patients with bladder cancer. In: Journal of Urology. 1986 ; Vol. 136, No. 4. pp. 815-819.
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