Flow cytometry monitoring of drug resistance in human tumor cells

Awtar Krishan

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Recent studies have identified a family of glycoproteins which moulate cellular transport of antibiotics, alkaloids and drugs used in cancer chemotherapy. By facilitating efflux of drugs from the intracellular domain, these proteins reduce cytotoxicity and thus confer drug resistance. With the availability of antibodies raised against these phenotypic markers of drug resistance, immunohistochemistry and flow cytometry has been used to study their distribution and expression in normal and tumor cells. As some of the drugs used in cancer chemotherapy and other dyes which are substrates for this efflux pump are fluorescent, laser flow cytometry can be used for rapid quantitation of cellular retention, efflux and heterogeneity in drug transport of a tumor cell population. This method can also be used to screen drugs which may block efflux of a chemotherpeutic drug and thus increase chemosensitivity of a drug resistant tumor. In the present report flow cytometric methods for the study of drug transport and its modulation in tumor cells are discussed.

Original languageEnglish
Pages (from-to)55-60
Number of pages6
JournalMethods in Cell Science
Volume24
Issue number1-3
DOIs
StatePublished - Dec 1 2002
Externally publishedYes

Fingerprint

Flow cytometry
Drug Resistance
Tumors
Flow Cytometry
Cells
Chemotherapy
Monitoring
Pharmaceutical Preparations
Neoplasms
Alkaloids
Glycoproteins
Antibiotics
Cytotoxicity
Antibodies
Dyes
Modulation
Availability
Pumps
Proteins
Drug Therapy

Keywords

  • Drug resistance
  • Drug transport
  • Flow cytometry
  • Multiple drug resistance

ASJC Scopus subject areas

  • Cell Biology

Cite this

Flow cytometry monitoring of drug resistance in human tumor cells. / Krishan, Awtar.

In: Methods in Cell Science, Vol. 24, No. 1-3, 01.12.2002, p. 55-60.

Research output: Contribution to journalArticle

Krishan, Awtar. / Flow cytometry monitoring of drug resistance in human tumor cells. In: Methods in Cell Science. 2002 ; Vol. 24, No. 1-3. pp. 55-60.
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