Abstract
Recent studies have identified a family of glycoproteins which moulate cellular transport of antibiotics, alkaloids and drugs used in cancer chemotherapy. By facilitating efflux of drugs from the intracellular domain, these proteins reduce cytotoxicity and thus confer drug resistance. With the availability of antibodies raised against these phenotypic markers of drug resistance, immunohistochemistry and flow cytometry has been used to study their distribution and expression in normal and tumor cells. As some of the drugs used in cancer chemotherapy and other dyes which are substrates for this efflux pump are fluorescent, laser flow cytometry can be used for rapid quantitation of cellular retention, efflux and heterogeneity in drug transport of a tumor cell population. This method can also be used to screen drugs which may block efflux of a chemotherpeutic drug and thus increase chemosensitivity of a drug resistant tumor. In the present report flow cytometric methods for the study of drug transport and its modulation in tumor cells are discussed.
| Original language | English |
|---|---|
| Pages (from-to) | 55-60 |
| Number of pages | 6 |
| Journal | Methods in Cell Science |
| Volume | 24 |
| Issue number | 1-3 |
| DOIs | |
| State | Published - Dec 1 2002 |
| Externally published | Yes |
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Keywords
- Drug resistance
- Drug transport
- Flow cytometry
- Multiple drug resistance
ASJC Scopus subject areas
- Cell Biology
Cite this
Flow cytometry monitoring of drug resistance in human tumor cells. / Krishan, Awtar.
In: Methods in Cell Science, Vol. 24, No. 1-3, 01.12.2002, p. 55-60.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Flow cytometry monitoring of drug resistance in human tumor cells
AU - Krishan, Awtar
PY - 2002/12/1
Y1 - 2002/12/1
N2 - Recent studies have identified a family of glycoproteins which moulate cellular transport of antibiotics, alkaloids and drugs used in cancer chemotherapy. By facilitating efflux of drugs from the intracellular domain, these proteins reduce cytotoxicity and thus confer drug resistance. With the availability of antibodies raised against these phenotypic markers of drug resistance, immunohistochemistry and flow cytometry has been used to study their distribution and expression in normal and tumor cells. As some of the drugs used in cancer chemotherapy and other dyes which are substrates for this efflux pump are fluorescent, laser flow cytometry can be used for rapid quantitation of cellular retention, efflux and heterogeneity in drug transport of a tumor cell population. This method can also be used to screen drugs which may block efflux of a chemotherpeutic drug and thus increase chemosensitivity of a drug resistant tumor. In the present report flow cytometric methods for the study of drug transport and its modulation in tumor cells are discussed.
AB - Recent studies have identified a family of glycoproteins which moulate cellular transport of antibiotics, alkaloids and drugs used in cancer chemotherapy. By facilitating efflux of drugs from the intracellular domain, these proteins reduce cytotoxicity and thus confer drug resistance. With the availability of antibodies raised against these phenotypic markers of drug resistance, immunohistochemistry and flow cytometry has been used to study their distribution and expression in normal and tumor cells. As some of the drugs used in cancer chemotherapy and other dyes which are substrates for this efflux pump are fluorescent, laser flow cytometry can be used for rapid quantitation of cellular retention, efflux and heterogeneity in drug transport of a tumor cell population. This method can also be used to screen drugs which may block efflux of a chemotherpeutic drug and thus increase chemosensitivity of a drug resistant tumor. In the present report flow cytometric methods for the study of drug transport and its modulation in tumor cells are discussed.
KW - Drug resistance
KW - Drug transport
KW - Flow cytometry
KW - Multiple drug resistance
UR - http://www.scopus.com/inward/record.url?scp=0038692955&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0038692955&partnerID=8YFLogxK
U2 - 10.1023/A:1024185612997
DO - 10.1023/A:1024185612997
M3 - Article
C2 - 12815291
AN - SCOPUS:0038692955
VL - 24
SP - 55
EP - 60
JO - Cytotechnology
JF - Cytotechnology
SN - 0920-9069
IS - 1-3
ER -