Abstract
Laser flow cytometry (FCM) provides an excellent tool for monitoring of markers and mechanisms of diagnostic and prognostic significance in oncology. Recently, FCM has been used to monitor cellular drug transport in tumor cells (Cytometry 8:306; 1987). FCM cannot only analyze cellular drug transport characteristics but also screen other drugs which may modify these characteristics and thus increase cellular sensitivity to cancer chemotherapy. These studies have especially focussed on the phenomena of drug efflux which is believed to be responsible for resistance of tumor cells to a variety of natural products used in chemotherapy. Besides drug efflux and its modulation, FCM has been used for monitoring of markers and mechanisms involved in cellular detoxification of cancer drugs (Biochemical Pharm. 39:723; 1989). Several recent studies have shown that cellular glutathiones and the related enzymes may be elevated in certain tumor cells. Several protocols have been developed to reduce cellular glutathione levels in the hope of ameliorating drug resistance. With the recent availability of fluorochromes for GSH measurement and antibodies to some of the related enzymes, FCM can be used to monitor markers and mechanisms involved in drug detoxification. This review techniques and data on FCM monitoring of P-glycoprotein (believed to act as an efflux pump), cellular drug retention and its modulation, cellular content glutathione and some of the related enzymes.
| Original language | English (US) |
|---|---|
| Number of pages | 1 |
| Journal | Annals of biomedical engineering |
| Volume | 19 |
| Issue number | 5 |
| State | Published - Dec 1 1991 |
| Event | 1991 Annual Fall Meeting of the Biomedical Engineering Society - Charlottesville, VA, USA Duration: Oct 12 1991 → Oct 14 1991 |
ASJC Scopus subject areas
- Biomedical Engineering