Flow cytometric monitoring of hormone receptor expression in human solid tumors

Awtar Krishan

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Hormone receptor expression in human breast and prostate tumors is of diagnostic and therapeutic importance. With the availability of anti-estrogen, androgen and progesterone antibodies, immunohistochemistry has become a standard tool for determination of receptor expression in human tumor biopsies. However, this method is dependent on examination of a small number of cells under a microscope and the data obtained in most cases is not quantitative. As most of the commercially used anti-hormone antibodies have nuclear specificity, we have developed methods for isolation and antigen unmasking of nuclei from formalin fixed/paraffin embedded archival human tumors. After immunostaining with the antibodies and propidium iodide (for DNA content and cell cycle analysis), nuclei are analyzed by multiparametric laser flow cytometry for hormone receptor expression, DNA content, aneuploidy and cell cycle determination. These multiparametric methods are especially important for retrospective studies seeking to correlate hormone receptor expression with clinical response to anti-hormonal therapy of human breast and prostate tumors.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsD.L. Farkas, R.C. Leif
Pages211-217
Number of pages7
Volume4622
DOIs
StatePublished - 2002
Externally publishedYes
EventOptical Diagnostics of Living Cells V - San Jose, CA, United States
Duration: Jan 23 2002Jan 25 2002

Other

OtherOptical Diagnostics of Living Cells V
CountryUnited States
CitySan Jose, CA
Period1/23/021/25/02

Fingerprint

hormones
Hormones
Tumors
tumors
antibodies
Antibodies
Monitoring
breast
DNA
deoxyribonucleic acid
Cells
estrogens
cycles
nuclei
cytometry
Flow cytometry
Biopsy
paraffins
antigens
Antigens

Keywords

  • Androgen
  • Estrogen
  • Flow cytometry
  • Hormone receptors
  • Progesterone

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Krishan, A. (2002). Flow cytometric monitoring of hormone receptor expression in human solid tumors. In D. L. Farkas, & R. C. Leif (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 4622, pp. 211-217) https://doi.org/10.1117/12.468346

Flow cytometric monitoring of hormone receptor expression in human solid tumors. / Krishan, Awtar.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / D.L. Farkas; R.C. Leif. Vol. 4622 2002. p. 211-217.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Krishan, A 2002, Flow cytometric monitoring of hormone receptor expression in human solid tumors. in DL Farkas & RC Leif (eds), Proceedings of SPIE - The International Society for Optical Engineering. vol. 4622, pp. 211-217, Optical Diagnostics of Living Cells V, San Jose, CA, United States, 1/23/02. https://doi.org/10.1117/12.468346
Krishan A. Flow cytometric monitoring of hormone receptor expression in human solid tumors. In Farkas DL, Leif RC, editors, Proceedings of SPIE - The International Society for Optical Engineering. Vol. 4622. 2002. p. 211-217 https://doi.org/10.1117/12.468346
Krishan, Awtar. / Flow cytometric monitoring of hormone receptor expression in human solid tumors. Proceedings of SPIE - The International Society for Optical Engineering. editor / D.L. Farkas ; R.C. Leif. Vol. 4622 2002. pp. 211-217
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