Flow cytometric monitoring of cellular anthracycline accumulation in murine leukemic cells

Awtar Krishan, Antonieta Sauerteig, Kristie Gordon, Carol Swinkin

Research output: Contribution to journalArticle

54 Scopus citations

Abstract

Cellular accumulation of daunorubicin (DNR), N-trifluoroacetyl-adriamycin-14-valerate and THP-Adriamycin (THP-ADR) in doxorubicin sensitive and resistant murine leukemic P388 cells was studied with laser excited flow cytometry. Appearance of DNR fluorescence in P388/S cells was rapid in contrast to that of P388/R cells. A comparison of P388/S and P388//R cells incubated for 20-30 min showed that DNR fluorescence in P388/R cells was one-sixth that of P388/S cells. In contrast, the difference between fluorescence value of P388/S and P388/R cells similarly incubated with N-trifluoroacetyl-adriamycin-14-valerate or THP-ADR was less than 2-fold. Chlorpromazine, verapamil, and trifluoperazine increased the cellular accumulation and cytoxicity of DNR and THP-ADR but had no major effect on N-trifluoroacetyl-adriamycin-14-valerate fluorescence or cytotoxicity in P388/R cells. Fluorometric and soft agar assays confirmed the data on the effect of these modulators on drug accumulation obtained by the more rapid method of laser flow cytometry.

Original languageEnglish (US)
Pages (from-to)1768-1773
Number of pages6
JournalCancer Research
Volume46
StatePublished - Apr 1 1986

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Krishan, A., Sauerteig, A., Gordon, K., & Swinkin, C. (1986). Flow cytometric monitoring of cellular anthracycline accumulation in murine leukemic cells. Cancer Research, 46, 1768-1773.