Flow cytometric differentiation of abnormal and normal plasma cells in the bone marrow in patients with multiple myeloma and its precursor diseases

Prashant R. Tembhare, Constance M. Yuan, David Venzon, Raul Braylan, Neha Korde, Elisabet Manasanch, Diamond Zuchlinsky, Katherine Calvo, Roger Kurlander, Manisha Bhutani, Nishant Tageja, Irina Maric, Marcia Mulquin, Mark Roschewski, Mary Kwok, David Liewehr, Ola Landgren, Maryalice Stetler-Stevenson

Research output: Contribution to journalArticlepeer-review

56 Scopus citations

Abstract

Flow cytometric (FC) enumeration of abnormal plasma cells (APCs) for diagnosis and prognostication of plasma cell dyscrasias (PCD) is challenging. We studied antigen expression in normal plasma cells (NPC) (N= 34) and APC in a series of unselected PCD (N= 59). NPC subpopulations often demonstrated CD19(-), CD20(+), CD45(-) or dim and CD56(+), an immunophenotype observed in PCD. However abnormal CD81 was only observed in APCs (APC detection sensitivity 95%; specificity 100%). We evaluated differences in antigen expression patterns among MGUS (N= 14), SMM ( N= 35) and MM (N= 10), finding the combination of CD45 and CD56 helpful in differentiating MGUS from SMM and MM (p= 0.0002).

Original languageEnglish (US)
Pages (from-to)371-376
Number of pages6
JournalLeukemia Research
Volume38
Issue number3
DOIs
StatePublished - Mar 2014
Externally publishedYes

Keywords

  • CD81
  • Monoclonal gammopathy of uncertain significance
  • Multiple myeloma
  • Normal plasma cell immunophenotype
  • Smoldering multiple myeloma

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

Fingerprint

Dive into the research topics of 'Flow cytometric differentiation of abnormal and normal plasma cells in the bone marrow in patients with multiple myeloma and its precursor diseases'. Together they form a unique fingerprint.

Cite this