Flow cytometric analysis of the response of the R3327-G rat prostatic adenocarcinoma to endocrine manipulation

Alan Pollack, C. B. Bagwell, Norman L Block, G. L. Irvin, A. J. Claflin, B. J. Stover

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The techinqiue of flow cytometric DNA histogram analysis (FCM) shows there to be two distinct cell populations (diploid vs aneuploid) in the poorly differenterial R3327-G rat prostatic adenocarcinoma. The following study compares tumor weight measurements with several FCM computer-based methods designed to determine rapidly the proliferative status of tumors. Hypophysectomy, bilateral adrenalectomy, orchiectomy, sham operations, or diethylstilbestrol treatments were initiated when the tumors were palpable (day 21) and continued until the tumors were excised (day 52). Hypophysectomy, orchiectomy, adrenalectomy, and diethylstilbestrol treatments all resulted in significant inhibition by tumor weight. Quantitation of the percantage of mild-S phase aneuploid cells by summation gave the best correlation with tumor weight. Tumors grown in hypophysectomized, orchiectomized, adrenalectomized, or diethylstilbestrol-treated animals showed a significant reduction in the proportion of mid-S phase cells as compared with controls. The calculation of the percentage of all aneuploid cells was significantly reduced in hypophysectomy, orchiectomy, and diethylstilbestrol-treated animals. However, tumors grown in adrenalectomized animals were not significantly different from controls by this method. Adrenalectony was found to be the least effective form of therapy, and this was reflected in all of the parameters measured. These data show that FCM analysis may be useful in the quantitation of prostatic carcinoma response to therapy.

Original languageEnglish
Pages (from-to)389-398
Number of pages10
JournalJournal of Surgical Oncology
Volume18
Issue number4
StatePublished - Dec 1 1981

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Diethylstilbestrol
Adenocarcinoma
Hypophysectomy
Orchiectomy
Aneuploidy
Tumor Burden
Adrenalectomy
Neoplasms
S Phase
Diploidy
Carcinoma
DNA
Therapeutics
Population

ASJC Scopus subject areas

  • Surgery
  • Oncology

Cite this

Pollack, A., Bagwell, C. B., Block, N. L., Irvin, G. L., Claflin, A. J., & Stover, B. J. (1981). Flow cytometric analysis of the response of the R3327-G rat prostatic adenocarcinoma to endocrine manipulation. Journal of Surgical Oncology, 18(4), 389-398.

Flow cytometric analysis of the response of the R3327-G rat prostatic adenocarcinoma to endocrine manipulation. / Pollack, Alan; Bagwell, C. B.; Block, Norman L; Irvin, G. L.; Claflin, A. J.; Stover, B. J.

In: Journal of Surgical Oncology, Vol. 18, No. 4, 01.12.1981, p. 389-398.

Research output: Contribution to journalArticle

Pollack, A, Bagwell, CB, Block, NL, Irvin, GL, Claflin, AJ & Stover, BJ 1981, 'Flow cytometric analysis of the response of the R3327-G rat prostatic adenocarcinoma to endocrine manipulation', Journal of Surgical Oncology, vol. 18, no. 4, pp. 389-398.
Pollack, Alan ; Bagwell, C. B. ; Block, Norman L ; Irvin, G. L. ; Claflin, A. J. ; Stover, B. J. / Flow cytometric analysis of the response of the R3327-G rat prostatic adenocarcinoma to endocrine manipulation. In: Journal of Surgical Oncology. 1981 ; Vol. 18, No. 4. pp. 389-398.
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