Flow cytometric analysis of R3327 rat prostate adenocarcinoma grown in vivo and in vitro

A. J. Claflin, A. Pollack, T. Malinin, N. L. Block, G. L. Irvin

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

The Dunning R3327 transplantable prostate adenocarcinoma in the Copenhagen rat is an acceptable model for the human disease. The G-subline (a rapidly growing carcinoma) and the H-subline (a slow-growing, well-differentiated adenocarcinoma) represent the extremes of differentiation and growth rate of this tumor. Both sublines were found to have one population that was diploid and a second aneuploid population that was hyperdiploid in DNA content. The percentage of hyperdiploid cells was significantly higher in R3327-G tumors than in R3327-H tumors. The tumor cell population ratios were stable in vivo, but the in vitro culture conditions supported only cells with diploid DNA content following four to five subcultures. These predominantly diploid cultured cells, when injected into intact male rats, resulted in tumors that had both diploid and aneuploid cells.

Original languageEnglish (US)
Pages (from-to)79-87
Number of pages9
JournalJournal of the National Cancer Institute
Volume69
Issue number1
StatePublished - Oct 25 1982

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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