Abstract
Small cell osteosarcoma and mesenchymal chondrosarcoma are 2 primary bone tumors with a small round blue cell component, which can mimic the appearance of Ewing sarcoma. Distinguishing these tumors from each other on biopsy material is important clinically, as optimal therapy differs according to the tumor type. However, separating these entities on morphology alone can be challenging. FLI-1 has been described to be a useful marker for Ewing sarcoma, particularly when hematolymphoid markers are negative. In small cell osteosarcoma and mesenchymal chondrosarcoma, the FLI-1 staining pattern has not been adequately characterized. Using a monoclonal FLI-1 antibody, nuclear immunoreactivity in tumor cells was evaluated in 10 small cell osteosarcomas, 10 mesenchymal chondrosarcomas, and 8 Ewing sarcomas, together with a number of other small, round, blue cell tumors. None of the small cell osteosarcomas or mesenchymal chondrosarcomas exhibited FLI-1 staining in the tumor cells, in contrast to the positive nuclear FLI-1 staining in the stromal endothelial cells. In comparison, 6 of the 8 Ewing sarcomas showed moderate-to-strong nuclear FLI-1 staining of the tumor cells in addition to strong staining of the stromal endothelial cell nuclei. With the exception of lymphoblastic lymphomas, FLI-1 positivity was not seen in the other small round blue cell tumors examined. These findings show that, in contrast to Ewing sarcoma, small cell osteosarcoma and mesenchymal chondrosarcoma lack FLI-1 immunoreactivity. FLI-1 is therefore useful in the differential diagnosis of small round blue cell tumors of the bone.
| Original language | English |
|---|---|
| Pages (from-to) | 233-238 |
| Number of pages | 6 |
| Journal | Applied Immunohistochemistry and Molecular Morphology |
| Volume | 19 |
| Issue number | 3 |
| DOIs | |
| State | Published - May 1 2011 |
| Externally published | Yes |
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Keywords
- ewing sarcoma
- FLI-1
- immunohistochemistry
- mesenchymal chondrosarcoma
- small cell osteosarcoma
- small round blue cell tumor
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Medical Laboratory Technology
- Histology
Cite this
FLI-1 distinguishes ewing sarcoma from small cell osteosarcoma and mesenchymal chondrosarcoma. / Lee, Anna F.; Hayes, Malcolm M.; Lebrun, David; Espinosa, Inigo; Nielsen, G. Petur; Rosenberg, Andrew; Lee, Cheng Han.
In: Applied Immunohistochemistry and Molecular Morphology, Vol. 19, No. 3, 01.05.2011, p. 233-238.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - FLI-1 distinguishes ewing sarcoma from small cell osteosarcoma and mesenchymal chondrosarcoma
AU - Lee, Anna F.
AU - Hayes, Malcolm M.
AU - Lebrun, David
AU - Espinosa, Inigo
AU - Nielsen, G. Petur
AU - Rosenberg, Andrew
AU - Lee, Cheng Han
PY - 2011/5/1
Y1 - 2011/5/1
N2 - Small cell osteosarcoma and mesenchymal chondrosarcoma are 2 primary bone tumors with a small round blue cell component, which can mimic the appearance of Ewing sarcoma. Distinguishing these tumors from each other on biopsy material is important clinically, as optimal therapy differs according to the tumor type. However, separating these entities on morphology alone can be challenging. FLI-1 has been described to be a useful marker for Ewing sarcoma, particularly when hematolymphoid markers are negative. In small cell osteosarcoma and mesenchymal chondrosarcoma, the FLI-1 staining pattern has not been adequately characterized. Using a monoclonal FLI-1 antibody, nuclear immunoreactivity in tumor cells was evaluated in 10 small cell osteosarcomas, 10 mesenchymal chondrosarcomas, and 8 Ewing sarcomas, together with a number of other small, round, blue cell tumors. None of the small cell osteosarcomas or mesenchymal chondrosarcomas exhibited FLI-1 staining in the tumor cells, in contrast to the positive nuclear FLI-1 staining in the stromal endothelial cells. In comparison, 6 of the 8 Ewing sarcomas showed moderate-to-strong nuclear FLI-1 staining of the tumor cells in addition to strong staining of the stromal endothelial cell nuclei. With the exception of lymphoblastic lymphomas, FLI-1 positivity was not seen in the other small round blue cell tumors examined. These findings show that, in contrast to Ewing sarcoma, small cell osteosarcoma and mesenchymal chondrosarcoma lack FLI-1 immunoreactivity. FLI-1 is therefore useful in the differential diagnosis of small round blue cell tumors of the bone.
AB - Small cell osteosarcoma and mesenchymal chondrosarcoma are 2 primary bone tumors with a small round blue cell component, which can mimic the appearance of Ewing sarcoma. Distinguishing these tumors from each other on biopsy material is important clinically, as optimal therapy differs according to the tumor type. However, separating these entities on morphology alone can be challenging. FLI-1 has been described to be a useful marker for Ewing sarcoma, particularly when hematolymphoid markers are negative. In small cell osteosarcoma and mesenchymal chondrosarcoma, the FLI-1 staining pattern has not been adequately characterized. Using a monoclonal FLI-1 antibody, nuclear immunoreactivity in tumor cells was evaluated in 10 small cell osteosarcomas, 10 mesenchymal chondrosarcomas, and 8 Ewing sarcomas, together with a number of other small, round, blue cell tumors. None of the small cell osteosarcomas or mesenchymal chondrosarcomas exhibited FLI-1 staining in the tumor cells, in contrast to the positive nuclear FLI-1 staining in the stromal endothelial cells. In comparison, 6 of the 8 Ewing sarcomas showed moderate-to-strong nuclear FLI-1 staining of the tumor cells in addition to strong staining of the stromal endothelial cell nuclei. With the exception of lymphoblastic lymphomas, FLI-1 positivity was not seen in the other small round blue cell tumors examined. These findings show that, in contrast to Ewing sarcoma, small cell osteosarcoma and mesenchymal chondrosarcoma lack FLI-1 immunoreactivity. FLI-1 is therefore useful in the differential diagnosis of small round blue cell tumors of the bone.
KW - ewing sarcoma
KW - FLI-1
KW - immunohistochemistry
KW - mesenchymal chondrosarcoma
KW - small cell osteosarcoma
KW - small round blue cell tumor
UR - http://www.scopus.com/inward/record.url?scp=79955068165&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79955068165&partnerID=8YFLogxK
U2 - 10.1097/PAI.0b013e3181fd6697
DO - 10.1097/PAI.0b013e3181fd6697
M3 - Article
C2 - 21084965
AN - SCOPUS:79955068165
VL - 19
SP - 233
EP - 238
JO - Applied Immunohistochemistry and Molecular Morphology
JF - Applied Immunohistochemistry and Molecular Morphology
SN - 1541-2016
IS - 3
ER -