Fission yeast MO25 protein is localized at SPB and septum and is essential for cell morphogenesis

Muneyoshi Kanai, Kazunori Kume, Kohji Miyahara, Keisuke Sakai, Keigo Nakamura, Klaus Leonhard, David J. Wiley, Fulvia Verde, Takashi Toda, Dai Hirata

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Cell morphogenesis is of fundamental significance in all eukaryotes for development, differentiation, and cell proliferation. In fission yeast, Drosophila Furry-like Mor2 plays an essential role in cell morphogenesis in concert with the NDR/Tricornered kinase Orb6. Mutations of these genes result in the loss of cell polarity. Here we show that the conserved proteins, MO25-like Pmo25, GC kinase Nak1, Mor2, and Orb6, constitute a morphogenesis network that is important for polarity control and cell separation. Intriguingly, Pmo25 was localized at the mitotic spindle pole bodies (SPBs) and then underwent translocation to the dividing medial region upon cytokinesis. Pmo25 formed a complex with Nak1 and was required for both the localization and kinase activity of Nak1. Pmo25 and Nak1 in turn were essential for Orb6 kinase activity. Further, the Pmo25 localization at the SPBs and the Nak1-Orb6 kinase activities during interphase were under the control of the Cdc7 and Sid1 kinases in the septation initiation network (SIN), suggesting a functional linkage between SIN and the network for cell morphogenesis/separation following cytokinesis.

Original languageEnglish
Pages (from-to)3012-3025
Number of pages14
JournalEMBO Journal
Volume24
Issue number17
DOIs
StatePublished - Sep 1 2005

Fingerprint

Schizosaccharomyces pombe Proteins
Spindle Pole Bodies
Morphogenesis
Poles
Phosphotransferases
Cytokinesis
Cell Separation
Cell Polarity
Spindle Apparatus
Schizosaccharomyces
Interphase
Cell proliferation
Eukaryota
Yeast
Drosophila
Genes
Cell Proliferation
Fungal Proteins
Mutation
Proteins

Keywords

  • Cell morphogenesis
  • MO25
  • Mor2
  • SIN
  • SPB

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Kanai, M., Kume, K., Miyahara, K., Sakai, K., Nakamura, K., Leonhard, K., ... Hirata, D. (2005). Fission yeast MO25 protein is localized at SPB and septum and is essential for cell morphogenesis. EMBO Journal, 24(17), 3012-3025. https://doi.org/10.1038/sj.emboj.7600782

Fission yeast MO25 protein is localized at SPB and septum and is essential for cell morphogenesis. / Kanai, Muneyoshi; Kume, Kazunori; Miyahara, Kohji; Sakai, Keisuke; Nakamura, Keigo; Leonhard, Klaus; Wiley, David J.; Verde, Fulvia; Toda, Takashi; Hirata, Dai.

In: EMBO Journal, Vol. 24, No. 17, 01.09.2005, p. 3012-3025.

Research output: Contribution to journalArticle

Kanai, M, Kume, K, Miyahara, K, Sakai, K, Nakamura, K, Leonhard, K, Wiley, DJ, Verde, F, Toda, T & Hirata, D 2005, 'Fission yeast MO25 protein is localized at SPB and septum and is essential for cell morphogenesis', EMBO Journal, vol. 24, no. 17, pp. 3012-3025. https://doi.org/10.1038/sj.emboj.7600782
Kanai M, Kume K, Miyahara K, Sakai K, Nakamura K, Leonhard K et al. Fission yeast MO25 protein is localized at SPB and septum and is essential for cell morphogenesis. EMBO Journal. 2005 Sep 1;24(17):3012-3025. https://doi.org/10.1038/sj.emboj.7600782
Kanai, Muneyoshi ; Kume, Kazunori ; Miyahara, Kohji ; Sakai, Keisuke ; Nakamura, Keigo ; Leonhard, Klaus ; Wiley, David J. ; Verde, Fulvia ; Toda, Takashi ; Hirata, Dai. / Fission yeast MO25 protein is localized at SPB and septum and is essential for cell morphogenesis. In: EMBO Journal. 2005 ; Vol. 24, No. 17. pp. 3012-3025.
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