Fission yeast cell morphogenesis

Identification of new genes and analysis of their role during the cell cycle

Fulvia Verde, Juan Mata, Paul Nurse

Research output: Contribution to journalArticle

160 Citations (Scopus)

Abstract

To identify new genes involved in the control of cell morphogenesis in the fission yeast Schizosaccharomyces pombe we have visually screened for temperature-sensitive mutants that show defects in cell morphology. We have isolated and characterized 64 mutants defining 19 independent genes, 10 of which have not been previously described. One class of mutants, defining 12 orb genes, become round and show a complete loss of cell polarity. A second class of mutants exhibits branched or bent morphologies. These mutants show defects in either selection of the growth site, defining two tea genes, or in the maintenance of growth direction, defining five ban genes. Immunofluorescence analysis of these morphological mutants shows defects in the organization of the microtubule and actin cytoskeleton. These defects include shortened, bundled, and asymmetrically localized microtubules and enlarged and mislocalized actin patches. Analysis of the mutant phenotypes has allowed us to order the genes into four groups according to their function during the cell cycle: genes required for the maintenance of cell polarity throughout the cell cycle: genes necessary only for the reestablishment of cell polarity after mitosis and not for maintaining cell polarity once it is established; genes essential for the transition from monopolar to bipolar growth and genes that serve as 'polarity markers'.

Original languageEnglish
Pages (from-to)1529-1538
Number of pages10
JournalJournal of Cell Biology
Volume131
Issue number6 I
DOIs
StatePublished - Dec 1 1995
Externally publishedYes

Fingerprint

Schizosaccharomyces
Morphogenesis
Cell Polarity
Cell Cycle
Genes
cdc Genes
Microtubules
Growth
Maintenance
Gene Order
Essential Genes
Tea
Actin Cytoskeleton
Mitosis
Fluorescent Antibody Technique
Actins
Phenotype
Temperature

ASJC Scopus subject areas

  • Cell Biology

Cite this

Fission yeast cell morphogenesis : Identification of new genes and analysis of their role during the cell cycle. / Verde, Fulvia; Mata, Juan; Nurse, Paul.

In: Journal of Cell Biology, Vol. 131, No. 6 I, 01.12.1995, p. 1529-1538.

Research output: Contribution to journalArticle

@article{44dbbfa940f34fe38ce43d20da264ea1,
title = "Fission yeast cell morphogenesis: Identification of new genes and analysis of their role during the cell cycle",
abstract = "To identify new genes involved in the control of cell morphogenesis in the fission yeast Schizosaccharomyces pombe we have visually screened for temperature-sensitive mutants that show defects in cell morphology. We have isolated and characterized 64 mutants defining 19 independent genes, 10 of which have not been previously described. One class of mutants, defining 12 orb genes, become round and show a complete loss of cell polarity. A second class of mutants exhibits branched or bent morphologies. These mutants show defects in either selection of the growth site, defining two tea genes, or in the maintenance of growth direction, defining five ban genes. Immunofluorescence analysis of these morphological mutants shows defects in the organization of the microtubule and actin cytoskeleton. These defects include shortened, bundled, and asymmetrically localized microtubules and enlarged and mislocalized actin patches. Analysis of the mutant phenotypes has allowed us to order the genes into four groups according to their function during the cell cycle: genes required for the maintenance of cell polarity throughout the cell cycle: genes necessary only for the reestablishment of cell polarity after mitosis and not for maintaining cell polarity once it is established; genes essential for the transition from monopolar to bipolar growth and genes that serve as 'polarity markers'.",
author = "Fulvia Verde and Juan Mata and Paul Nurse",
year = "1995",
month = "12",
day = "1",
doi = "10.1083/jcb.131.6.1529",
language = "English",
volume = "131",
pages = "1529--1538",
journal = "Journal of Cell Biology",
issn = "0021-9525",
publisher = "Rockefeller University Press",
number = "6 I",

}

TY - JOUR

T1 - Fission yeast cell morphogenesis

T2 - Identification of new genes and analysis of their role during the cell cycle

AU - Verde, Fulvia

AU - Mata, Juan

AU - Nurse, Paul

PY - 1995/12/1

Y1 - 1995/12/1

N2 - To identify new genes involved in the control of cell morphogenesis in the fission yeast Schizosaccharomyces pombe we have visually screened for temperature-sensitive mutants that show defects in cell morphology. We have isolated and characterized 64 mutants defining 19 independent genes, 10 of which have not been previously described. One class of mutants, defining 12 orb genes, become round and show a complete loss of cell polarity. A second class of mutants exhibits branched or bent morphologies. These mutants show defects in either selection of the growth site, defining two tea genes, or in the maintenance of growth direction, defining five ban genes. Immunofluorescence analysis of these morphological mutants shows defects in the organization of the microtubule and actin cytoskeleton. These defects include shortened, bundled, and asymmetrically localized microtubules and enlarged and mislocalized actin patches. Analysis of the mutant phenotypes has allowed us to order the genes into four groups according to their function during the cell cycle: genes required for the maintenance of cell polarity throughout the cell cycle: genes necessary only for the reestablishment of cell polarity after mitosis and not for maintaining cell polarity once it is established; genes essential for the transition from monopolar to bipolar growth and genes that serve as 'polarity markers'.

AB - To identify new genes involved in the control of cell morphogenesis in the fission yeast Schizosaccharomyces pombe we have visually screened for temperature-sensitive mutants that show defects in cell morphology. We have isolated and characterized 64 mutants defining 19 independent genes, 10 of which have not been previously described. One class of mutants, defining 12 orb genes, become round and show a complete loss of cell polarity. A second class of mutants exhibits branched or bent morphologies. These mutants show defects in either selection of the growth site, defining two tea genes, or in the maintenance of growth direction, defining five ban genes. Immunofluorescence analysis of these morphological mutants shows defects in the organization of the microtubule and actin cytoskeleton. These defects include shortened, bundled, and asymmetrically localized microtubules and enlarged and mislocalized actin patches. Analysis of the mutant phenotypes has allowed us to order the genes into four groups according to their function during the cell cycle: genes required for the maintenance of cell polarity throughout the cell cycle: genes necessary only for the reestablishment of cell polarity after mitosis and not for maintaining cell polarity once it is established; genes essential for the transition from monopolar to bipolar growth and genes that serve as 'polarity markers'.

UR - http://www.scopus.com/inward/record.url?scp=0029610382&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029610382&partnerID=8YFLogxK

U2 - 10.1083/jcb.131.6.1529

DO - 10.1083/jcb.131.6.1529

M3 - Article

VL - 131

SP - 1529

EP - 1538

JO - Journal of Cell Biology

JF - Journal of Cell Biology

SN - 0021-9525

IS - 6 I

ER -