Cytochalasin B, a fungal metabolite, induces multinucleate cell formation by preventing the separation of daughter cells following cell division. These cells contain large amounts of rough endoplasmic reticulum, annulate lamellae, Golgi membranes, and other cell organelles in their cytoplasm. Microtubules are seen in the mitotic spindle and in the cytoplasm of these relatively nonmotile cells. In many drug-treated cells basal bodies (presumably formed from centrioles) and various stages of ciliogenesis are seen. In some cells fully developed cilia projecting from the cell surface are observed. An apparent stimulated production of l-cell virus-like particles is seen in these multinucleated cells.
ASJC Scopus subject areas
- Molecular Biology