Field evaluation of an enzyme-linked immunosorbent assay (ELISA) for Plasmodium falciparum sporozoite detection in anopheline mosquitoes from Kenya

J. C. Beier, P. V. Perkins, R. A. Wirtz, R. E. Whitmire, M. Mugambi, W. T. Hockmeyer

Research output: Contribution to journalArticle

67 Scopus citations


An enzyme-linked immunosorbent assay (ELISA) using a monoclonal antibody that recognizes a repetitive epitope on the circumsporozoite protein of Plasmodium falciparum was used in Kenya to assess malaria infections in Anopheles gambiae s.l. and An. funestus. The ELISA confirmed that 88% of 44 sporozoite-positive gland dissections were P. falciparum. The ELISA infection rate of 18.6% (n=736) for individually tested mosquitoes for both species was significantly higher than the 10.4% (n=537) salivary gland sporozoite rate determined by dissection. This difference was due to ELISA detection of medium and large sized oocysts on the midguts of infected mosquitoes which did not contain salivary gland sporozoites. From a series of 379 Anopheles that were cut at the thorax, ELISA tests on 'head' and 'body' portions showed that 29.5% of 95 positive mosquitoes contained circumsporozoite antigen in the body portion in the absence of salivary gland infections. This field evaluation demonstrates that the ELISA can most accurately be used to estimate sporozoite rates by cutting mosquitoes at the thorax and testing anterior portions.

Original languageEnglish (US)
Pages (from-to)459-468
Number of pages10
JournalAmerican Journal of Tropical Medicine and Hygiene
Issue number3
StatePublished - Jan 1 1987


ASJC Scopus subject areas

  • Parasitology
  • Virology
  • Infectious Diseases

Cite this