TY - JOUR
T1 - Ferrociphenol lipid nanocapsule delivery by mesenchymal stromal cells in brain tumor therapy
AU - Roger, Mathilde
AU - Clavreul, Anne
AU - Huynh, Ngoc Trinh
AU - Passirani, Catherine
AU - Schiller, Paul
AU - Vessires, Anne
AU - Montero-Menei, Claudia
AU - Menei, Philippe
N1 - Funding Information:
This work was supported by the “Association en Avant la Vie” and by VFK Krebsforschung gGmbH (Berlin, Allemagne) . We thank Dr Jean Paul Jacob from “la coordination hospitalière des prélèvements d’organes et de tissus” (CHU, Angers, France). We are also grateful to Pierre Legras and Jérôme Roux (Service commun d’animalerie Hospitalo-Universitaire, Angers, France) for the facilities provided.
PY - 2012/2/14
Y1 - 2012/2/14
N2 - The prognosis of patients with malignant glioma remains extremely poor despite surgery and improvements in radio- and chemo-therapies. Thus, treatment strategies that specifically target these tumors have the potential to greatly improve therapeutic outcomes. "Marrow-isolated adult multilineage inducible" cells (MIAMI cells) are a subpopulation of mesenchymal stromal cells (MSCs) which possess the ability to migrate to brain tumors. We have previously shown that MIAMI cells were able to efficiently incorporate lipid nanocapsules (LNCs) without altering either their stem cell properties or their migration capacity. In this study, we assessed whether the cytotoxic effects of MIAMI cells loaded with LNCs containing an organometallic complex (ferrociphenol or Fc-diOH) could be used to treat brain tumors. The results showed that MIAMI cells internalized Fc-diOH-LNCs and that this internalization did not induce MIAMI cell death. Furthermore, Fc-diOH-LNC-loaded MIAMI cells produced a cytotoxic effect on U87MG glioma cells in vitro. This cytotoxic effect was validated in vivo after intratumoral injection of Fc-diOH-LNC-loaded MIAMI cells in a heterotopic U87MG glioma model in nude mice. These promising results open up a new field of treatment in which cellular vehicles and nanoparticles can be combined to treat brain tumors.
AB - The prognosis of patients with malignant glioma remains extremely poor despite surgery and improvements in radio- and chemo-therapies. Thus, treatment strategies that specifically target these tumors have the potential to greatly improve therapeutic outcomes. "Marrow-isolated adult multilineage inducible" cells (MIAMI cells) are a subpopulation of mesenchymal stromal cells (MSCs) which possess the ability to migrate to brain tumors. We have previously shown that MIAMI cells were able to efficiently incorporate lipid nanocapsules (LNCs) without altering either their stem cell properties or their migration capacity. In this study, we assessed whether the cytotoxic effects of MIAMI cells loaded with LNCs containing an organometallic complex (ferrociphenol or Fc-diOH) could be used to treat brain tumors. The results showed that MIAMI cells internalized Fc-diOH-LNCs and that this internalization did not induce MIAMI cell death. Furthermore, Fc-diOH-LNC-loaded MIAMI cells produced a cytotoxic effect on U87MG glioma cells in vitro. This cytotoxic effect was validated in vivo after intratumoral injection of Fc-diOH-LNC-loaded MIAMI cells in a heterotopic U87MG glioma model in nude mice. These promising results open up a new field of treatment in which cellular vehicles and nanoparticles can be combined to treat brain tumors.
KW - Glioma
KW - Mesenchymal stromal cells
KW - Nanocarriers
KW - Organometallic compound
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U2 - 10.1016/j.ijpharm.2011.04.058
DO - 10.1016/j.ijpharm.2011.04.058
M3 - Article
C2 - 21554935
AN - SCOPUS:84855804108
VL - 423
SP - 63
EP - 68
JO - International Journal of Pharmaceutics
JF - International Journal of Pharmaceutics
SN - 0378-5173
IS - 1
ER -