Fatty acid unsaturation increases expression and capping of murine lymphocyte CD44 and CD45

Michael D. Peck, Zhiming Li, Tieran Han, Wenzhi Wang, Wenche Jy, Yeon Ahn, Vincent A. Ziboh, Arthur J. Chu, Lilly Y W Bourguignon

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We studied the effect of incubating murine lymphocytes with cis- unsaturated fatty acids on expression and capping of CD44 and CD45. Lymphocytes were incubated with stearic (18:0) or oleic (18:1ω-9) acid bound to bovine serum albumin (BSA). After incubation with rat anti-CD44 or anti- CD45 monoclonal antibodies and then with fluorescent-labeled anti-rat antibody, mean fluorescence intensity (FI) was measured by using flow cytometry. Capping was measured after warming and fixation in paraformaldehyde. Steady-state fluorescence anisotropy (r(s)) was measured after the cells had been incubated with trimethylammonium-diphenylhexatriene. Incubation with oleic acid, but not stearic acid or BSA alone, was associated with an increase in FI of CD44. Expression of CD45, however, was increased by both stearic and oleic acids to the same degree over BSA controls. CD44 and CD45 capping were both increased by incubation with oleic acid. R(s) was decreased in cells incubated with oleic acid, suggesting an increase in membrane fluidity. We conclude that incubation with oleic acid increases expression of CD44 and increases capping of both CD44 and CD45. These findings were confirmed in feeding experiments, in which r(s) was reduced and CD44 capping increased by polyunsaturated fatty acid diets.

Original languageEnglish
Pages (from-to)616-622
Number of pages7
JournalNutrition
Volume12
Issue number9
DOIs
StatePublished - Sep 1 1996

Fingerprint

Oleic Acid
Fatty Acids
Bovine Serum Albumin
Lymphocytes
Unsaturated Fatty Acids
Fluorescence
Stearic Acids
Membrane Fluidity
Fluorescence Polarization
Anti-Idiotypic Antibodies
Flow Cytometry
Monoclonal Antibodies
Diet
Acids

Keywords

  • CD44 antigens
  • CD45 antigens
  • lipids
  • lymphocytes
  • membrane fluidity
  • membrane lipids
  • membrane proteins
  • membranes
  • mice
  • oleic acid

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Surgery
  • Medicine (miscellaneous)

Cite this

Peck, M. D., Li, Z., Han, T., Wang, W., Jy, W., Ahn, Y., ... Bourguignon, L. Y. W. (1996). Fatty acid unsaturation increases expression and capping of murine lymphocyte CD44 and CD45. Nutrition, 12(9), 616-622. https://doi.org/10.1016/S0899-9007(96)00177-3

Fatty acid unsaturation increases expression and capping of murine lymphocyte CD44 and CD45. / Peck, Michael D.; Li, Zhiming; Han, Tieran; Wang, Wenzhi; Jy, Wenche; Ahn, Yeon; Ziboh, Vincent A.; Chu, Arthur J.; Bourguignon, Lilly Y W.

In: Nutrition, Vol. 12, No. 9, 01.09.1996, p. 616-622.

Research output: Contribution to journalArticle

Peck, MD, Li, Z, Han, T, Wang, W, Jy, W, Ahn, Y, Ziboh, VA, Chu, AJ & Bourguignon, LYW 1996, 'Fatty acid unsaturation increases expression and capping of murine lymphocyte CD44 and CD45', Nutrition, vol. 12, no. 9, pp. 616-622. https://doi.org/10.1016/S0899-9007(96)00177-3
Peck, Michael D. ; Li, Zhiming ; Han, Tieran ; Wang, Wenzhi ; Jy, Wenche ; Ahn, Yeon ; Ziboh, Vincent A. ; Chu, Arthur J. ; Bourguignon, Lilly Y W. / Fatty acid unsaturation increases expression and capping of murine lymphocyte CD44 and CD45. In: Nutrition. 1996 ; Vol. 12, No. 9. pp. 616-622.
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