Abstract
We synthesized a photoswitchable fluorescent probe incorporating a BODIPY fluorophore and an oxazine photochrome within the same molecular skeleton. The selective excitation of the BODIPY component at visible wavelengths is accompanied by the emission of light in the form of fluorescence. However, the illumination of the sample at ultraviolet wavelengths opens reversibly the oxazine ring and activates the intramolecular transfer of energy from the fluorophore to the photochrome with concomitant fluorescence quenching. As a result, the emission of this fluorophore-photochrome dyad can be modulated on a microsecond time scale for hundreds of switching cycles, relying on the interplay of two exciting beams. Our operating principles for fast fluorescence photoswitching with excellent fatigue resistance can lead to the development of valuable probes for the super-resolution imaging of biological samples.
| Original language | English |
|---|---|
| Pages (from-to) | 1690-1693 |
| Number of pages | 4 |
| Journal | Journal of Physical Chemistry Letters |
| Volume | 1 |
| Issue number | 11 |
| DOIs | |
| State | Published - Jun 3 2010 |
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ASJC Scopus subject areas
- Materials Science(all)
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Fast fluorescence photoswitching in a bodipy-oxazine dyad with excellent fatigue resistance. / Deniz, Erhan; Sortino, Salvatore; Raymo, Francisco.
In: Journal of Physical Chemistry Letters, Vol. 1, No. 11, 03.06.2010, p. 1690-1693.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Fast fluorescence photoswitching in a bodipy-oxazine dyad with excellent fatigue resistance
AU - Deniz, Erhan
AU - Sortino, Salvatore
AU - Raymo, Francisco
PY - 2010/6/3
Y1 - 2010/6/3
N2 - We synthesized a photoswitchable fluorescent probe incorporating a BODIPY fluorophore and an oxazine photochrome within the same molecular skeleton. The selective excitation of the BODIPY component at visible wavelengths is accompanied by the emission of light in the form of fluorescence. However, the illumination of the sample at ultraviolet wavelengths opens reversibly the oxazine ring and activates the intramolecular transfer of energy from the fluorophore to the photochrome with concomitant fluorescence quenching. As a result, the emission of this fluorophore-photochrome dyad can be modulated on a microsecond time scale for hundreds of switching cycles, relying on the interplay of two exciting beams. Our operating principles for fast fluorescence photoswitching with excellent fatigue resistance can lead to the development of valuable probes for the super-resolution imaging of biological samples.
AB - We synthesized a photoswitchable fluorescent probe incorporating a BODIPY fluorophore and an oxazine photochrome within the same molecular skeleton. The selective excitation of the BODIPY component at visible wavelengths is accompanied by the emission of light in the form of fluorescence. However, the illumination of the sample at ultraviolet wavelengths opens reversibly the oxazine ring and activates the intramolecular transfer of energy from the fluorophore to the photochrome with concomitant fluorescence quenching. As a result, the emission of this fluorophore-photochrome dyad can be modulated on a microsecond time scale for hundreds of switching cycles, relying on the interplay of two exciting beams. Our operating principles for fast fluorescence photoswitching with excellent fatigue resistance can lead to the development of valuable probes for the super-resolution imaging of biological samples.
UR - http://www.scopus.com/inward/record.url?scp=77953186777&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77953186777&partnerID=8YFLogxK
U2 - 10.1021/jz100510u
DO - 10.1021/jz100510u
M3 - Article
AN - SCOPUS:77953186777
VL - 1
SP - 1690
EP - 1693
JO - Journal of Physical Chemistry Letters
JF - Journal of Physical Chemistry Letters
SN - 1948-7185
IS - 11
ER -