Fast fluorescence photoswitching in a bodipy-oxazine dyad with excellent fatigue resistance

Erhan Deniz, Salvatore Sortino, Francisco Raymo

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

We synthesized a photoswitchable fluorescent probe incorporating a BODIPY fluorophore and an oxazine photochrome within the same molecular skeleton. The selective excitation of the BODIPY component at visible wavelengths is accompanied by the emission of light in the form of fluorescence. However, the illumination of the sample at ultraviolet wavelengths opens reversibly the oxazine ring and activates the intramolecular transfer of energy from the fluorophore to the photochrome with concomitant fluorescence quenching. As a result, the emission of this fluorophore-photochrome dyad can be modulated on a microsecond time scale for hundreds of switching cycles, relying on the interplay of two exciting beams. Our operating principles for fast fluorescence photoswitching with excellent fatigue resistance can lead to the development of valuable probes for the super-resolution imaging of biological samples.

Original languageEnglish
Pages (from-to)1690-1693
Number of pages4
JournalJournal of Physical Chemistry Letters
Volume1
Issue number11
DOIs
StatePublished - Jun 3 2010

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Oxazines
Fluorophores
Fluorescence
Fatigue of materials
fluorescence
Wavelength
probes
Fluorescent Dyes
musculoskeletal system
wavelengths
Quenching
Lighting
illumination
quenching
Imaging techniques
cycles
rings
excitation
4,4-difluoro-4-bora-3a,4a-diaza-s-indacene
energy

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Fast fluorescence photoswitching in a bodipy-oxazine dyad with excellent fatigue resistance. / Deniz, Erhan; Sortino, Salvatore; Raymo, Francisco.

In: Journal of Physical Chemistry Letters, Vol. 1, No. 11, 03.06.2010, p. 1690-1693.

Research output: Contribution to journalArticle

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