Far-Red Photoactivatable BODIPYs for the Super-Resolution Imaging of Live Cells

Yang Zhang, Ki Hee Song, Sicheng Tang, Laura Ravelo, Janet Cusido, Cheng Sun, Hao F. Zhang, Francisco Raymo

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

The photoinduced disconnection of an oxazine heterocycle from a borondipyrromethene (BODIPY) chromophore activates bright far-red fluorescence. The high brightness of the product and the lack of autofluorescence in this spectral region allow its detection at the single-molecule level within the organelles of live cells. Indeed, these photoactivatable fluorophores localize in lysosomal compartments and remain covalently immobilized within these organelles. The suppression of diffusion allows the reiterative reconstruction of subdiffraction images and the visualization of the labeled organelles with excellent localization precision. Thus, the combination of photochemical, photophysical and structural properties designed into our fluorophores enable the visualization of live cells with a spatial resolution that is inaccessible to conventional fluorescence imaging.

Original languageEnglish (US)
Pages (from-to)12741-12745
Number of pages5
JournalJournal of the American Chemical Society
Volume140
Issue number40
DOIs
StatePublished - Oct 10 2018

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ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Zhang, Y., Song, K. H., Tang, S., Ravelo, L., Cusido, J., Sun, C., Zhang, H. F., & Raymo, F. (2018). Far-Red Photoactivatable BODIPYs for the Super-Resolution Imaging of Live Cells. Journal of the American Chemical Society, 140(40), 12741-12745. https://doi.org/10.1021/jacs.8b09099