Faithful expression of GFP from the PLCβ2 promoter in a functional class of taste receptor cells

Joung Woul Kim, Craig Roberts, Yutaka Maruyama, Stephanie Berg, Stephen D Roper, Nirupa Chaudhari

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Phospholipase C-type β2 (PLCβ2) is expressed in a subset of cells within mammalian taste buds. This enzyme is involved in the transduction of sweet, bitter, and umami stimuli and thus is believed to be a marker for gustatory sensory receptor cells. We have developed transgenic mice expressing green fluorescent protein (GFP) under the control of the PLCβ2 promoter to enable one to identify these cells and record their physiological activity in living preparations. Expression of GFP (especially in lines with more than one copy integrated) is strong enough to be detected in intact tissue preparations using epifluorescence microscopy. By immunohistochemistry, we confirmed that the overwhelming majority of cells expressing GFP are those that endogenously express PLCβ2. Expression of the GFP transgene in circumvallate papillae occurs at about the same time during development as endogenous PLCβ2 expression. When loaded with a calcium-sensitive dye in situ, GFP-positive taste cells produce typical Ca2+ responses to a taste stimulus, the bitter compound cycloheximide. These PLCβ2 promoter-GFP transgenic lines promise to be useful for studying taste transduction, sensory signal processing, and taste bud development.

Original languageEnglish
Pages (from-to)213-219
Number of pages7
JournalChemical Senses
Volume31
Issue number3
DOIs
StatePublished - Mar 1 2006

Fingerprint

phospholipase C
Type C Phospholipases
Protein C
Green Fluorescent Proteins
green fluorescent protein
promoter regions
Taste Buds
palps
cells
bitter-tasting compounds
genetically modified organisms
sensory receptors
umami
calcium
cycloheximide
Sensory Receptor Cells
Cycloheximide
Transgenes
Transgenic Mice
transgenes

Keywords

  • GFP
  • Mouse
  • PLCβ2
  • Taste bud
  • Taste-specific promoter
  • Transgenic

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Neuroscience(all)
  • Physiology
  • Physiology (medical)
  • Behavioral Neuroscience

Cite this

Faithful expression of GFP from the PLCβ2 promoter in a functional class of taste receptor cells. / Kim, Joung Woul; Roberts, Craig; Maruyama, Yutaka; Berg, Stephanie; Roper, Stephen D; Chaudhari, Nirupa.

In: Chemical Senses, Vol. 31, No. 3, 01.03.2006, p. 213-219.

Research output: Contribution to journalArticle

Kim, Joung Woul ; Roberts, Craig ; Maruyama, Yutaka ; Berg, Stephanie ; Roper, Stephen D ; Chaudhari, Nirupa. / Faithful expression of GFP from the PLCβ2 promoter in a functional class of taste receptor cells. In: Chemical Senses. 2006 ; Vol. 31, No. 3. pp. 213-219.
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