Faecal pollution along the southeastern coast of Florida and insight into the use of pepper mild mottle virus as an indicator

E. M. Symonds, C. Sinigalliano, M. Gidley, W. Ahmed, S. M. McQuaig-Ulrich, M. Breitbart

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

Aims: To identify faecal pollution along the southeastern Florida coast and determine the performance of a reverse transcription-quantitative polymerase chain reaction (RT-qPCR) method for pepper mild mottle virus (PMMoV). Methods and Results: In 2014, bimonthly surface water samples were collected from inlets, exposed to runoff and septic seepage, and coastal sites, exposed to ocean outfalls. Analysis of culturable enterococci and a suite of microbial source tracking (MST) markers (BacHum, CowM2, DogBact, HF183, HPyV, PMMoV) revealed faecal pollution, primarily of human origin, at all sites. Since PMMoV was detected more frequently than other MST markers, the process limits of quantification (undiluted to 10−2 dilution) and detection (10−2 dilution) for the RT-qPCR method were determined by seeding untreated wastewater into the coastal waters. Simulated quantitative microbial risk assessment, employing human norovirus as a reference pathogen, calculated a 0·286 median risk of gastrointestinal illness associated with the PMMoV limit of detection. Conclusions: All sites met the U.S. EPA recreational water criteria, despite detection of domestic wastewater-associated MST markers. PMMoV correlated only with human-associated MST markers. Significance and Impact of the Study: This study demonstrated that PMMoV is an important domestic wastewater-associated marker that should be included in the MST toolbox; therefore, future studies should thoroughly investigate the health risks associated with its detection and quantification in environmental waters.

Original languageEnglish (US)
Pages (from-to)1469-1481
Number of pages13
JournalJournal of Applied Microbiology
Volume121
Issue number5
DOIs
StatePublished - Nov 1 2016

Keywords

  • enteric viruses
  • microbial source tracking
  • ocean outfall
  • quantitative microbial risk assessment
  • wastewater
  • water quality

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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