Factors That Affect Human Islet Isolation

Y. Sakuma, Camillo Ricordi, A. Miki, T. Yamamoto, A. Pileggi, A. Khan, Rodolfo Alejandro, Luca A Inverardi, H. Ichii

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

More than 10,000 IEQ/kg recipient weight of islets is often necessary to achieve insulin independence in patients with type 1 diabetes mellitus. Several studies have identified high donor body mass index (BMI) and pancreas size as important factors for the success of human islet isolation. However, the donor shortage underscores the need to improve isolation outcomes from lower BMI pancreas donors and/or small pancreata. The aim of this study was to identify the critical factors that affect isolation outcome. We analyzed the data from 207 isolations performed from 2002 to 2006 with respect to donor characteristics, pancreas condition, and processing variables. More than 3000 IEQ/g pancreas weight was considered to be an acceptable isolation outcome. This goal was obtained from donors with a BMI >30 kg/m2 (P = .002). The pancreatic surface integrity was also a significant factor (P = .02). Moreover, longer digestion times (P = .04) and a greater proportion of trapped islets negatively affected success rates (P = .004). As previously reported, pancreata from high BMI donors were suitable for islet isolation and transplantation, as they yielded higher total islet particle numbers and higher IEQ/g. Although BMI and pancreas size are not controllable due to the organ donor shortage, factors such as pancreatic surface integrity, shorter digestion time, and lower proportions of trapped islets were found to be significant to obtain higher success rates. The development of better protocols and systematic training of processing/procurement teams will be of assistance to increase the number of successful human islet isolations.

Original languageEnglish
Pages (from-to)343-345
Number of pages3
JournalTransplantation Proceedings
Volume40
Issue number2
DOIs
StatePublished - Mar 1 2008

Fingerprint

Pancreas
Tissue Donors
Body Mass Index
Digestion
Weights and Measures
Islets of Langerhans Transplantation
Type 1 Diabetes Mellitus
Insulin

ASJC Scopus subject areas

  • Surgery
  • Transplantation

Cite this

Sakuma, Y., Ricordi, C., Miki, A., Yamamoto, T., Pileggi, A., Khan, A., ... Ichii, H. (2008). Factors That Affect Human Islet Isolation. Transplantation Proceedings, 40(2), 343-345. https://doi.org/10.1016/j.transproceed.2007.12.019

Factors That Affect Human Islet Isolation. / Sakuma, Y.; Ricordi, Camillo; Miki, A.; Yamamoto, T.; Pileggi, A.; Khan, A.; Alejandro, Rodolfo; Inverardi, Luca A; Ichii, H.

In: Transplantation Proceedings, Vol. 40, No. 2, 01.03.2008, p. 343-345.

Research output: Contribution to journalArticle

Sakuma, Y, Ricordi, C, Miki, A, Yamamoto, T, Pileggi, A, Khan, A, Alejandro, R, Inverardi, LA & Ichii, H 2008, 'Factors That Affect Human Islet Isolation', Transplantation Proceedings, vol. 40, no. 2, pp. 343-345. https://doi.org/10.1016/j.transproceed.2007.12.019
Sakuma, Y. ; Ricordi, Camillo ; Miki, A. ; Yamamoto, T. ; Pileggi, A. ; Khan, A. ; Alejandro, Rodolfo ; Inverardi, Luca A ; Ichii, H. / Factors That Affect Human Islet Isolation. In: Transplantation Proceedings. 2008 ; Vol. 40, No. 2. pp. 343-345.
@article{415369af596b4915af155d15d18b4eb2,
title = "Factors That Affect Human Islet Isolation",
abstract = "More than 10,000 IEQ/kg recipient weight of islets is often necessary to achieve insulin independence in patients with type 1 diabetes mellitus. Several studies have identified high donor body mass index (BMI) and pancreas size as important factors for the success of human islet isolation. However, the donor shortage underscores the need to improve isolation outcomes from lower BMI pancreas donors and/or small pancreata. The aim of this study was to identify the critical factors that affect isolation outcome. We analyzed the data from 207 isolations performed from 2002 to 2006 with respect to donor characteristics, pancreas condition, and processing variables. More than 3000 IEQ/g pancreas weight was considered to be an acceptable isolation outcome. This goal was obtained from donors with a BMI >30 kg/m2 (P = .002). The pancreatic surface integrity was also a significant factor (P = .02). Moreover, longer digestion times (P = .04) and a greater proportion of trapped islets negatively affected success rates (P = .004). As previously reported, pancreata from high BMI donors were suitable for islet isolation and transplantation, as they yielded higher total islet particle numbers and higher IEQ/g. Although BMI and pancreas size are not controllable due to the organ donor shortage, factors such as pancreatic surface integrity, shorter digestion time, and lower proportions of trapped islets were found to be significant to obtain higher success rates. The development of better protocols and systematic training of processing/procurement teams will be of assistance to increase the number of successful human islet isolations.",
author = "Y. Sakuma and Camillo Ricordi and A. Miki and T. Yamamoto and A. Pileggi and A. Khan and Rodolfo Alejandro and Inverardi, {Luca A} and H. Ichii",
year = "2008",
month = "3",
day = "1",
doi = "10.1016/j.transproceed.2007.12.019",
language = "English",
volume = "40",
pages = "343--345",
journal = "Transplantation Proceedings",
issn = "0041-1345",
publisher = "Elsevier USA",
number = "2",

}

TY - JOUR

T1 - Factors That Affect Human Islet Isolation

AU - Sakuma, Y.

AU - Ricordi, Camillo

AU - Miki, A.

AU - Yamamoto, T.

AU - Pileggi, A.

AU - Khan, A.

AU - Alejandro, Rodolfo

AU - Inverardi, Luca A

AU - Ichii, H.

PY - 2008/3/1

Y1 - 2008/3/1

N2 - More than 10,000 IEQ/kg recipient weight of islets is often necessary to achieve insulin independence in patients with type 1 diabetes mellitus. Several studies have identified high donor body mass index (BMI) and pancreas size as important factors for the success of human islet isolation. However, the donor shortage underscores the need to improve isolation outcomes from lower BMI pancreas donors and/or small pancreata. The aim of this study was to identify the critical factors that affect isolation outcome. We analyzed the data from 207 isolations performed from 2002 to 2006 with respect to donor characteristics, pancreas condition, and processing variables. More than 3000 IEQ/g pancreas weight was considered to be an acceptable isolation outcome. This goal was obtained from donors with a BMI >30 kg/m2 (P = .002). The pancreatic surface integrity was also a significant factor (P = .02). Moreover, longer digestion times (P = .04) and a greater proportion of trapped islets negatively affected success rates (P = .004). As previously reported, pancreata from high BMI donors were suitable for islet isolation and transplantation, as they yielded higher total islet particle numbers and higher IEQ/g. Although BMI and pancreas size are not controllable due to the organ donor shortage, factors such as pancreatic surface integrity, shorter digestion time, and lower proportions of trapped islets were found to be significant to obtain higher success rates. The development of better protocols and systematic training of processing/procurement teams will be of assistance to increase the number of successful human islet isolations.

AB - More than 10,000 IEQ/kg recipient weight of islets is often necessary to achieve insulin independence in patients with type 1 diabetes mellitus. Several studies have identified high donor body mass index (BMI) and pancreas size as important factors for the success of human islet isolation. However, the donor shortage underscores the need to improve isolation outcomes from lower BMI pancreas donors and/or small pancreata. The aim of this study was to identify the critical factors that affect isolation outcome. We analyzed the data from 207 isolations performed from 2002 to 2006 with respect to donor characteristics, pancreas condition, and processing variables. More than 3000 IEQ/g pancreas weight was considered to be an acceptable isolation outcome. This goal was obtained from donors with a BMI >30 kg/m2 (P = .002). The pancreatic surface integrity was also a significant factor (P = .02). Moreover, longer digestion times (P = .04) and a greater proportion of trapped islets negatively affected success rates (P = .004). As previously reported, pancreata from high BMI donors were suitable for islet isolation and transplantation, as they yielded higher total islet particle numbers and higher IEQ/g. Although BMI and pancreas size are not controllable due to the organ donor shortage, factors such as pancreatic surface integrity, shorter digestion time, and lower proportions of trapped islets were found to be significant to obtain higher success rates. The development of better protocols and systematic training of processing/procurement teams will be of assistance to increase the number of successful human islet isolations.

UR - http://www.scopus.com/inward/record.url?scp=40949127890&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=40949127890&partnerID=8YFLogxK

U2 - 10.1016/j.transproceed.2007.12.019

DO - 10.1016/j.transproceed.2007.12.019

M3 - Article

C2 - 18374062

AN - SCOPUS:40949127890

VL - 40

SP - 343

EP - 345

JO - Transplantation Proceedings

JF - Transplantation Proceedings

SN - 0041-1345

IS - 2

ER -