Factors affecting DSAEK graft lenticle adhesion: An in vitro experimental study

Pravin K. Vaddavalli, Vasilios F. Diakonis, Ana P. Canto, Vardhaman P. Kankariya, Rajeev R. Pappuru, Marco Ruggeri, Michael R. Banitt, George D. Kymionis, Sonia H Yoo

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Purpose: The aim of this study was to evaluate different factors that affect Descemet stripping automated endothelial keratoplasty (DSAEK) donor graft lenticle adhesion to the recipient cornea. Methods: This experimental study included 10 eye bank recipient corneas and 10 donor DSAEK lenticles. Recipient corneas were mounted on an artificial anterior chamber (AC), whereas donor lenticles were placed beneath the host cornea. Using optical coherence tomography and imaging software, the interface gap (IG) between the donor and recipient cornea was quantified to evaluate the effect of variations in AC air fill pressure, AC air fill duration, corneal massage, and corneal venting incisions on DSAEK donor graft lenticle adhesion. Results: Different intraocular pressures (IOP) under air for the same time intervals, do not significantly correlate with the IG; nevertheless, it was noticed that the IG decreases as the IOP increases. With respect to the magnitude of AC IOP, there was no statistically significant difference when comparing 10 mm Hg with 30 mm Hg and assessing IG (P = 0.4). Complete air-fluid exchange resulted in significantly higher IG when compared with AC air bubble of 10 and 30 mm Hg that was sustained for 1 hour (P < 0.05). Furthermore, corneal surface massage did not facilitate DSAEK graft adhesion (P = 0.59). Finally, paracentral venting incisions followed by interface fluid aspiration seemed to significantly decrease the IG (P = 0.014). Conclusions: Corneal venting incisions and higher AC IOP values seem to facilitate DSAEK donor graft lenticle adhesion to the recipient cornea.

Original languageEnglish
Pages (from-to)551-554
Number of pages4
JournalCornea
Volume33
Issue number6
DOIs
StatePublished - Jan 1 2014

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Descemet Stripping Endothelial Keratoplasty
Anterior Chamber
Cornea
Transplants
Intraocular Pressure
Air
Massage
Eye Banks
Air Pressure
Optical Imaging
Optical Coherence Tomography
In Vitro Techniques
Software

Keywords

  • complications
  • cornea
  • corneal transplantations
  • DSAEK

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Vaddavalli, P. K., Diakonis, V. F., Canto, A. P., Kankariya, V. P., Pappuru, R. R., Ruggeri, M., ... Yoo, S. H. (2014). Factors affecting DSAEK graft lenticle adhesion: An in vitro experimental study. Cornea, 33(6), 551-554. https://doi.org/10.1097/ICO.0000000000000125

Factors affecting DSAEK graft lenticle adhesion : An in vitro experimental study. / Vaddavalli, Pravin K.; Diakonis, Vasilios F.; Canto, Ana P.; Kankariya, Vardhaman P.; Pappuru, Rajeev R.; Ruggeri, Marco; Banitt, Michael R.; Kymionis, George D.; Yoo, Sonia H.

In: Cornea, Vol. 33, No. 6, 01.01.2014, p. 551-554.

Research output: Contribution to journalArticle

Vaddavalli, PK, Diakonis, VF, Canto, AP, Kankariya, VP, Pappuru, RR, Ruggeri, M, Banitt, MR, Kymionis, GD & Yoo, SH 2014, 'Factors affecting DSAEK graft lenticle adhesion: An in vitro experimental study', Cornea, vol. 33, no. 6, pp. 551-554. https://doi.org/10.1097/ICO.0000000000000125
Vaddavalli PK, Diakonis VF, Canto AP, Kankariya VP, Pappuru RR, Ruggeri M et al. Factors affecting DSAEK graft lenticle adhesion: An in vitro experimental study. Cornea. 2014 Jan 1;33(6):551-554. https://doi.org/10.1097/ICO.0000000000000125
Vaddavalli, Pravin K. ; Diakonis, Vasilios F. ; Canto, Ana P. ; Kankariya, Vardhaman P. ; Pappuru, Rajeev R. ; Ruggeri, Marco ; Banitt, Michael R. ; Kymionis, George D. ; Yoo, Sonia H. / Factors affecting DSAEK graft lenticle adhesion : An in vitro experimental study. In: Cornea. 2014 ; Vol. 33, No. 6. pp. 551-554.
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abstract = "Purpose: The aim of this study was to evaluate different factors that affect Descemet stripping automated endothelial keratoplasty (DSAEK) donor graft lenticle adhesion to the recipient cornea. Methods: This experimental study included 10 eye bank recipient corneas and 10 donor DSAEK lenticles. Recipient corneas were mounted on an artificial anterior chamber (AC), whereas donor lenticles were placed beneath the host cornea. Using optical coherence tomography and imaging software, the interface gap (IG) between the donor and recipient cornea was quantified to evaluate the effect of variations in AC air fill pressure, AC air fill duration, corneal massage, and corneal venting incisions on DSAEK donor graft lenticle adhesion. Results: Different intraocular pressures (IOP) under air for the same time intervals, do not significantly correlate with the IG; nevertheless, it was noticed that the IG decreases as the IOP increases. With respect to the magnitude of AC IOP, there was no statistically significant difference when comparing 10 mm Hg with 30 mm Hg and assessing IG (P = 0.4). Complete air-fluid exchange resulted in significantly higher IG when compared with AC air bubble of 10 and 30 mm Hg that was sustained for 1 hour (P < 0.05). Furthermore, corneal surface massage did not facilitate DSAEK graft adhesion (P = 0.59). Finally, paracentral venting incisions followed by interface fluid aspiration seemed to significantly decrease the IG (P = 0.014). Conclusions: Corneal venting incisions and higher AC IOP values seem to facilitate DSAEK donor graft lenticle adhesion to the recipient cornea.",
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AU - Kankariya, Vardhaman P.

AU - Pappuru, Rajeev R.

AU - Ruggeri, Marco

AU - Banitt, Michael R.

AU - Kymionis, George D.

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