Abstract
Design, fabrication, and characterization of a novel surface acoustic wave (SAW) biosensor in complementary metaloxide semiconductor (CMOS) technology are introduced. The biosensor employs a streptavidin/biotin-based five-layer immunoassay for detecting a prominent breast cancer biomarker, mammoglobin (hMAM). There is a growing demand to develop a sensitive and specific assay to detect biomarkers in serum that could be used in the early detection of breast cancer, determining prognosis and monitoring therapy. CMOS-SAW devices present a viable alternative to the existing biosensor technologies by providing higher sensitivity levels and better performance at low costs. Two architectures (circular and rectangular) were developed and respective tests were presented for performance comparison. The sensitivities of the devices were analyzed primarily based on center frequency shifts. A frequency sensitivity of 8.704 pg/Hz and a mass sensitivity of 2810.25 m$^{2} /$kg were obtained. Selectivity tests were carried out against bovine serum albumin. Experimental results indicate that it is possible to attach cancer biomarkers to functionalized CMOS-SAW sensor surfaces and selectively detect hMAM antigens with improved sensitivities, lowered costs, and increased repeatability of fabrication.
| Original language | English |
|---|---|
| Article number | 5345716 |
| Pages (from-to) | 62-73 |
| Number of pages | 12 |
| Journal | IEEE Transactions on Biomedical Circuits and Systems |
| Volume | 4 |
| Issue number | 1 |
| DOIs | |
| State | Published - Feb 1 2010 |
| Externally published | Yes |
Fingerprint
Keywords
- Biosensor
- Cancer
- Complementary metal–oxide semiconductor (CMOS)
- Microelectromechanical systems (MEMS)
- Surface acoustic wave (SAW)
ASJC Scopus subject areas
- Electrical and Electronic Engineering
- Biomedical Engineering
Cite this
Fabrication and characterization of a surface-acoustic-wave biosensor in cmos technology for cancer biomarker detection. / Tigli, Onur; Bivona, Louis; Berg, Patricia; Zaghloul, Mona E.
In: IEEE Transactions on Biomedical Circuits and Systems, Vol. 4, No. 1, 5345716, 01.02.2010, p. 62-73.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Fabrication and characterization of a surface-acoustic-wave biosensor in cmos technology for cancer biomarker detection
AU - Tigli, Onur
AU - Bivona, Louis
AU - Berg, Patricia
AU - Zaghloul, Mona E.
PY - 2010/2/1
Y1 - 2010/2/1
N2 - Design, fabrication, and characterization of a novel surface acoustic wave (SAW) biosensor in complementary metaloxide semiconductor (CMOS) technology are introduced. The biosensor employs a streptavidin/biotin-based five-layer immunoassay for detecting a prominent breast cancer biomarker, mammoglobin (hMAM). There is a growing demand to develop a sensitive and specific assay to detect biomarkers in serum that could be used in the early detection of breast cancer, determining prognosis and monitoring therapy. CMOS-SAW devices present a viable alternative to the existing biosensor technologies by providing higher sensitivity levels and better performance at low costs. Two architectures (circular and rectangular) were developed and respective tests were presented for performance comparison. The sensitivities of the devices were analyzed primarily based on center frequency shifts. A frequency sensitivity of 8.704 pg/Hz and a mass sensitivity of 2810.25 m$^{2} /$kg were obtained. Selectivity tests were carried out against bovine serum albumin. Experimental results indicate that it is possible to attach cancer biomarkers to functionalized CMOS-SAW sensor surfaces and selectively detect hMAM antigens with improved sensitivities, lowered costs, and increased repeatability of fabrication.
AB - Design, fabrication, and characterization of a novel surface acoustic wave (SAW) biosensor in complementary metaloxide semiconductor (CMOS) technology are introduced. The biosensor employs a streptavidin/biotin-based five-layer immunoassay for detecting a prominent breast cancer biomarker, mammoglobin (hMAM). There is a growing demand to develop a sensitive and specific assay to detect biomarkers in serum that could be used in the early detection of breast cancer, determining prognosis and monitoring therapy. CMOS-SAW devices present a viable alternative to the existing biosensor technologies by providing higher sensitivity levels and better performance at low costs. Two architectures (circular and rectangular) were developed and respective tests were presented for performance comparison. The sensitivities of the devices were analyzed primarily based on center frequency shifts. A frequency sensitivity of 8.704 pg/Hz and a mass sensitivity of 2810.25 m$^{2} /$kg were obtained. Selectivity tests were carried out against bovine serum albumin. Experimental results indicate that it is possible to attach cancer biomarkers to functionalized CMOS-SAW sensor surfaces and selectively detect hMAM antigens with improved sensitivities, lowered costs, and increased repeatability of fabrication.
KW - Biosensor
KW - Cancer
KW - Complementary metal–oxide semiconductor (CMOS)
KW - Microelectromechanical systems (MEMS)
KW - Surface acoustic wave (SAW)
UR - http://www.scopus.com/inward/record.url?scp=76649109219&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=76649109219&partnerID=8YFLogxK
U2 - 10.1109/TBCAS.2009.2033662
DO - 10.1109/TBCAS.2009.2033662
M3 - Article
C2 - 23853310
AN - SCOPUS:76649109219
VL - 4
SP - 62
EP - 73
JO - IEEE Transactions on Biomedical Circuits and Systems
JF - IEEE Transactions on Biomedical Circuits and Systems
SN - 1932-4545
IS - 1
M1 - 5345716
ER -