F11-receptor (F11R/JAM) mediates platelet adhesion to endothelial cells: Role in inflammatory thrombosis

Anna Babinska, Mamdouh H. Kedees, Humra Athar, Tahir Ahmed, Olcay Batuman, Yigal H. Ehrlich, M. Mahmood Hussain, Elizabeth Kornecki

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

The F11 receptor (F11R) is a cell adhesion molecule (CAM), member of the immunoglobulin superfamily found on the surface of human platelets, and determined to play a role in platelet aggregation, secretion, adhesion and spreading. The same molecule is present also at tight junctions of endothelial cells (EC) where it is known as JAM and acts as a CAM through homophilic interactions. The role of F11R/JAM in the interaction of platelets with endothelial cells was investigated in the current studies. We report here that washed human platelets adhere specifically to a matrix made of immobilized, recombinant sF11R. Furthermore, platelets adhere to cytokine- (TNF-α, INF-γ) stimulated human umbilical vein endothelial cells (HUVEC), and approximately 40-60% of the adhesive force is exerted by homophilic interactions between the F11R of platelets and EC. This is evidenced by the inhibition of platelet adhesion to endothelial cells by recombinant soluble form of the F11R, and by two F11R peptides with amino acid sequences of the N-terminal region, and in the 1st Ig fold of the F11R, respectively. This study suggests a role for F11R in the adhesion of platelets to cytokine-inflamed endothelial cells and thus in thrombosis and atherosclerosis induced in non-denuded blood vessels by inflammatory processes. Agents that block the F11R-mediated adhesion of platelets to EC may be of therapeutic value in controlling thrombosis and preventing heart attacks and stroke.

Original languageEnglish
Pages (from-to)843-850
Number of pages8
JournalThrombosis and Haemostasis
Volume88
Issue number5
StatePublished - Nov 1 2002
Externally publishedYes

Fingerprint

Junctional Adhesion Molecule A
Thrombosis
Blood Platelets
Endothelial Cells
Cell Adhesion Molecules
Cytokines
Tight Junctions
Human Umbilical Vein Endothelial Cells
Platelet Aggregation
Adhesives
Blood Vessels
Immunoglobulins
Amino Acid Sequence
Atherosclerosis
Stroke
Myocardial Infarction

Keywords

  • Adhesion
  • F11R peptides
  • Human platelet F11 receptor (F11R)
  • HUVEC
  • Junctional adhesion molecule (JAM)
  • Platelet aggregation

ASJC Scopus subject areas

  • Hematology

Cite this

Babinska, A., Kedees, M. H., Athar, H., Ahmed, T., Batuman, O., Ehrlich, Y. H., ... Kornecki, E. (2002). F11-receptor (F11R/JAM) mediates platelet adhesion to endothelial cells: Role in inflammatory thrombosis. Thrombosis and Haemostasis, 88(5), 843-850.

F11-receptor (F11R/JAM) mediates platelet adhesion to endothelial cells : Role in inflammatory thrombosis. / Babinska, Anna; Kedees, Mamdouh H.; Athar, Humra; Ahmed, Tahir; Batuman, Olcay; Ehrlich, Yigal H.; Hussain, M. Mahmood; Kornecki, Elizabeth.

In: Thrombosis and Haemostasis, Vol. 88, No. 5, 01.11.2002, p. 843-850.

Research output: Contribution to journalArticle

Babinska, A, Kedees, MH, Athar, H, Ahmed, T, Batuman, O, Ehrlich, YH, Hussain, MM & Kornecki, E 2002, 'F11-receptor (F11R/JAM) mediates platelet adhesion to endothelial cells: Role in inflammatory thrombosis', Thrombosis and Haemostasis, vol. 88, no. 5, pp. 843-850.
Babinska A, Kedees MH, Athar H, Ahmed T, Batuman O, Ehrlich YH et al. F11-receptor (F11R/JAM) mediates platelet adhesion to endothelial cells: Role in inflammatory thrombosis. Thrombosis and Haemostasis. 2002 Nov 1;88(5):843-850.
Babinska, Anna ; Kedees, Mamdouh H. ; Athar, Humra ; Ahmed, Tahir ; Batuman, Olcay ; Ehrlich, Yigal H. ; Hussain, M. Mahmood ; Kornecki, Elizabeth. / F11-receptor (F11R/JAM) mediates platelet adhesion to endothelial cells : Role in inflammatory thrombosis. In: Thrombosis and Haemostasis. 2002 ; Vol. 88, No. 5. pp. 843-850.
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AU - Ahmed, Tahir

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