Extremely low D/H ratios of photoproduced hydrogen by cyanobacteria

Yao Hua Luo, Leonel Steinberg, Shoichiro Suda, Shuzo Kumazawa, Akira Mitsui

Research output: Contribution to journalArticle

64 Scopus citations

Abstract

Cyanobacteria, having primary photosynthetic reactions similar to higher plants, are capable of producing large quantities of molecular hydrogen by nitrogenase and/or hydrogenase delivering electrons to hydrogen ions via ferredoxin or oxidation of NADPH. We measured the deuterium/hydrogen (D/H) ratios of the hydrogen gas photoproduced by Synechococcus sp. Miami BG 043511 and Anabaena sp. TU 37-1, and demonstrate that ΔD values of their hydrogen gas are extremely low (about - 600%) when compared with that of available water (-7%).This depletion gives a mean fractionation factor (a) of 0.43, which is similar to that calculated for hydrogen ions at equilibrium with water (0.35) and hydrogen produced by electrolysis of water (0.24) but significantly different from those of carbon bound hydrogens (>0.83). Thus hydrogen ions available for protonation of NADP+ may be extremely deuterium depleted. Our results may explain why D/H ratios of nitrated cellulose or lipids from most plants are always depleted relative to water available for photosynthesis.

Original languageEnglish (US)
Pages (from-to)897-900
Number of pages4
JournalPlant and Cell Physiology
Volume32
Issue number6
StatePublished - Sep 1 1991

Keywords

  • δD
  • Anabaena sp.
  • Deuterium-photoproduced hydrogen
  • Synechococcus sp

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Ecology
  • Cell Biology
  • Physiology
  • Plant Science

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  • Cite this

    Luo, Y. H., Steinberg, L., Suda, S., Kumazawa, S., & Mitsui, A. (1991). Extremely low D/H ratios of photoproduced hydrogen by cyanobacteria. Plant and Cell Physiology, 32(6), 897-900.