Extremely low D/H ratios of photoproduced hydrogen by cyanobacteria

Yao Hua Luo, Leonel Sternberg, Shoichiro Suda, Shuzo Kumazawa, Akira Mitsui

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Cyanobacteria, having primary photosynthetic reactions similar to higher plants, are capable of producing large quantities of molecular hydrogen by nitrogenase and/or hydrogenase delivering electrons to hydrogen ions via ferredoxin or oxidation of NADPH. We measured the deuterium/hydrogen (D/H) ratios of the hydrogen gas photoproduced by Synechococcus sp. Miami BG 043511 and Anabaena sp. TU 37-1, and demonstrate that ΔD values of their hydrogen gas are extremely low (about - 600%) when compared with that of available water (-7%).This depletion gives a mean fractionation factor (a) of 0.43, which is similar to that calculated for hydrogen ions at equilibrium with water (0.35) and hydrogen produced by electrolysis of water (0.24) but significantly different from those of carbon bound hydrogens (>0.83). Thus hydrogen ions available for protonation of NADP+ may be extremely deuterium depleted. Our results may explain why D/H ratios of nitrated cellulose or lipids from most plants are always depleted relative to water available for photosynthesis.

Original languageEnglish
Pages (from-to)897-900
Number of pages4
JournalPlant and Cell Physiology
Volume32
Issue number6
StatePublished - Sep 1 1991

Fingerprint

Cyanobacteria
deuterium
Deuterium
Hydrogen
hydrogen
cyanobacterium
hydrogen ions
Protons
Water
NADP
NADP (coenzyme)
water
Gases
gases
ferredoxin hydrogenase
Anabaena
Synechococcus
Electrolysis
Hydrogenase
Nitrogenase

Keywords

  • δD
  • Anabaena sp.
  • Deuterium-photoproduced hydrogen
  • Synechococcus sp

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Ecology
  • Cell Biology
  • Physiology
  • Plant Science

Cite this

Luo, Y. H., Sternberg, L., Suda, S., Kumazawa, S., & Mitsui, A. (1991). Extremely low D/H ratios of photoproduced hydrogen by cyanobacteria. Plant and Cell Physiology, 32(6), 897-900.

Extremely low D/H ratios of photoproduced hydrogen by cyanobacteria. / Luo, Yao Hua; Sternberg, Leonel; Suda, Shoichiro; Kumazawa, Shuzo; Mitsui, Akira.

In: Plant and Cell Physiology, Vol. 32, No. 6, 01.09.1991, p. 897-900.

Research output: Contribution to journalArticle

Luo, YH, Sternberg, L, Suda, S, Kumazawa, S & Mitsui, A 1991, 'Extremely low D/H ratios of photoproduced hydrogen by cyanobacteria', Plant and Cell Physiology, vol. 32, no. 6, pp. 897-900.
Luo YH, Sternberg L, Suda S, Kumazawa S, Mitsui A. Extremely low D/H ratios of photoproduced hydrogen by cyanobacteria. Plant and Cell Physiology. 1991 Sep 1;32(6):897-900.
Luo, Yao Hua ; Sternberg, Leonel ; Suda, Shoichiro ; Kumazawa, Shuzo ; Mitsui, Akira. / Extremely low D/H ratios of photoproduced hydrogen by cyanobacteria. In: Plant and Cell Physiology. 1991 ; Vol. 32, No. 6. pp. 897-900.
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AB - Cyanobacteria, having primary photosynthetic reactions similar to higher plants, are capable of producing large quantities of molecular hydrogen by nitrogenase and/or hydrogenase delivering electrons to hydrogen ions via ferredoxin or oxidation of NADPH. We measured the deuterium/hydrogen (D/H) ratios of the hydrogen gas photoproduced by Synechococcus sp. Miami BG 043511 and Anabaena sp. TU 37-1, and demonstrate that ΔD values of their hydrogen gas are extremely low (about - 600%) when compared with that of available water (-7%).This depletion gives a mean fractionation factor (a) of 0.43, which is similar to that calculated for hydrogen ions at equilibrium with water (0.35) and hydrogen produced by electrolysis of water (0.24) but significantly different from those of carbon bound hydrogens (>0.83). Thus hydrogen ions available for protonation of NADP+ may be extremely deuterium depleted. Our results may explain why D/H ratios of nitrated cellulose or lipids from most plants are always depleted relative to water available for photosynthesis.

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