Extracellular matrix molecules and cell adhesion molecules induce neurites through different mechanisms

John Bixby, Perseus Jhabvala

Research output: Contribution to journalArticle

103 Citations (Scopus)

Abstract

It has recently become clear that both extracellular matrix (ECM) glycoproteins and various cell adhesion molecules (CAMs) can promote neurite outgrowth from primary neurons, though little is known of the intracellular mechanisms through which these signals are transduced. We have previously obtained evidence that protein kinase C function is an important part of the neuronal response to laminin (Bixby, J. L. 1989. Neuron. 3:287-297). Because such CAMs as L1 (Lagenauer, C., and V. Lemmon. 1987. Proc. Natl. Acad. Sci. USA. 84:7753-7757) and N-cadherin (Bixby, J. L. and R. Zhang. 1990. J. Cell Biol. 110:1253-1260) can be purified and used as substrates to promote neurite growth, we have now tested whether the response to CAMs is similarly dependent on protein kinase C. We find that inhibition of protein kinase C inhibits growth on fibronectin or collagen as well as on laminin. In contrast, C kinase inhibition actually potentiates the initial growth response to L1 or N-cadherin. The later "phase" of outgrowth on both of these CAMs is inhibited, however. Additionally, phorbol esters, which have no effect on neurite growth when optimal laminin concentrations are used, potentiate growth even on optimal concentrations of L1 or N-cadherin. The results indicate that different intracellular mechanisms operate during initial process outgrowth on ECM substrates as compared to CAM substrates, and suggest that protein kinase C function is required for continued neurite growth on each of these glycoproteins.

Original languageEnglish
Pages (from-to)2725-2732
Number of pages8
JournalJournal of Cell Biology
Volume111
Issue number6 PART 1
StatePublished - Dec 1 1990

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Cell Adhesion Molecules
Neurites
Extracellular Matrix
Protein Kinase C
Laminin
Cadherins
Growth
Glycoproteins
Neural Cell Adhesion Molecule L1
Neurons
Phorbol Esters
Fibronectins
Phosphotransferases
Collagen

ASJC Scopus subject areas

  • Cell Biology

Cite this

Extracellular matrix molecules and cell adhesion molecules induce neurites through different mechanisms. / Bixby, John; Jhabvala, Perseus.

In: Journal of Cell Biology, Vol. 111, No. 6 PART 1, 01.12.1990, p. 2725-2732.

Research output: Contribution to journalArticle

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