Extracellular Ca2+ induces a rapid increase in cytoplasmic free Ca2+ in pancreatic β-cells

Thomas Nilsson, Per Arkhammar, Per Olof Berggren

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


Using pancreatic β-cells isolated from obese hyperglycemic mice, it was demonstrated that the addition of 5 mM extracellular Ca2+ evoked a rapid and transient increase in cytoplasmic free Ca2+ concentration ([Ca2+]i). The effect remained in the presence of D-600. Extracellular Ca2+ did not raise [Ca2+]i subsequent to emptying the inositol 1,4,5-trisphosphate (InsP3) sensitive pool by carbamylcholine stimulation, indicating that the pool released by extracellular Ca2+ is of similar origin. Stimulation with extracellular Ca2+ was accompanied by a pronounced insulin release. Our results suggest that the Ca2+-induced rise in [Ca2+]i is mediated through the formation of InsP3, a mechanism that might operate also in other types of cells.

Original languageEnglish (US)
Pages (from-to)152-158
Number of pages7
JournalBiochemical and biophysical research communications
Issue number1
StatePublished - Nov 30 1987

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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