Extracellular Ca2+ induces a rapid increase in cytoplasmic free Ca2+ in pancreatic β-cells

Thomas Nilsson, Per Arkhammar, Per Olof Berggren

Research output: Contribution to journalArticle

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Abstract

Using pancreatic β-cells isolated from obese hyperglycemic mice, it was demonstrated that the addition of 5 mM extracellular Ca2+ evoked a rapid and transient increase in cytoplasmic free Ca2+ concentration ([Ca2+]i). The effect remained in the presence of D-600. Extracellular Ca2+ did not raise [Ca2+]i subsequent to emptying the inositol 1,4,5-trisphosphate (InsP3) sensitive pool by carbamylcholine stimulation, indicating that the pool released by extracellular Ca2+ is of similar origin. Stimulation with extracellular Ca2+ was accompanied by a pronounced insulin release. Our results suggest that the Ca2+-induced rise in [Ca2+]i is mediated through the formation of InsP3, a mechanism that might operate also in other types of cells.

Original languageEnglish
Pages (from-to)152-158
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume149
Issue number1
DOIs
StatePublished - Nov 30 1987
Externally publishedYes

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Gallopamil
Obese Mice
Inositol 1,4,5-Trisphosphate
Carbachol
Insulin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Extracellular Ca2+ induces a rapid increase in cytoplasmic free Ca2+ in pancreatic β-cells. / Nilsson, Thomas; Arkhammar, Per; Berggren, Per Olof.

In: Biochemical and Biophysical Research Communications, Vol. 149, No. 1, 30.11.1987, p. 152-158.

Research output: Contribution to journalArticle

Nilsson, Thomas ; Arkhammar, Per ; Berggren, Per Olof. / Extracellular Ca2+ induces a rapid increase in cytoplasmic free Ca2+ in pancreatic β-cells. In: Biochemical and Biophysical Research Communications. 1987 ; Vol. 149, No. 1. pp. 152-158.
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