Expression profiling of gastric adenocarcinoma using cDNA array

Wa'el El-Rifai, Henry F. Frierson, Jeffrey C. Harper, Steven M. Powell, Sakari Knuutila

Research output: Contribution to journalArticle

88 Scopus citations

Abstract

To investigate the expression profile of gastric adenocarcinoma, cDNA array experiments were performed using Atlas Human Cancer 1.2 K Array (Clontech Laboratories, Palo Alto, CA) on nine xenografted and two primary gastric cancer samples. The expression of the tumor samples was compared to that of two normal gastric epithelial tissues. The expression pattern of the primary tumors was similar to that of xenografted tumors. The up-regulated genes had expression ratios ranging from 2.5 to 16, whereas the down-regulated genes had a range from -2.5 to -16. No variation in gene expression was detected in the analysis of the xenografted tumors versus the primary tumors, indicating that the xenografts represented the primary tumors well. Thirty-eight genes showed altered gene expression in 5 or more samples (>45%). Thirty-one genes were up-regulated and seven genes were down-regulated. The most abundantly up-regulated genes (ratio >5) included genes such as SI00A4, CDK4, MMPI4 and beta catenin. The GIF was markedly down-regulated (ratio < - 10). To confirm our findings, six genes (three up- and three down-regulated) were selected for semi-quantitative RT-PCR analysis. The RT-PCR results were consistent with the array findings. Our approach revealed that several genes are abnormally expressed in gastric cancer and found that genes known to interact in several common molecular pathway(s) were consistently altered.

Original languageEnglish (US)
Pages (from-to)832-838
Number of pages7
JournalInternational Journal of Cancer
Volume92
Issue number6
DOIs
StatePublished - Jun 15 2001
Externally publishedYes

Keywords

  • Gastric adenocarcinomas
  • Gene expression
  • Xenografis
  • cDNA arrays

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    El-Rifai, W., Frierson, H. F., Harper, J. C., Powell, S. M., & Knuutila, S. (2001). Expression profiling of gastric adenocarcinoma using cDNA array. International Journal of Cancer, 92(6), 832-838. https://doi.org/10.1002/ijc.1264