Expression parameters for target gene cloned in Escherichia coli in response to phosphate supply

Sanjoy K. Bhattacharya, Ashok K. Dubey

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Recombinant strain of E. coli 1727 overexpressed target gene at enhanced level when supplied with excess of inorganic phosphate. Rate of target gene expression, yield coefficient for target gene product and plasmid copy number increased significantly: 50%, 100% and 40% respectively at 125 mM excess phosphate. This was, however, accompanied by 26% decrease in specific growth rate and 30% in cellular growth yield coefficient.

Original languageEnglish (US)
Pages (from-to)1145-1148
Number of pages4
JournalBiotechnology Letters
Volume18
Issue number10
DOIs
StatePublished - Nov 20 1996
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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