Recombinant strain of E. coli 1727 overexpressed target gene at enhanced level when supplied with excess of inorganic phosphate. Rate of target gene expression, yield coefficient for target gene product and plasmid copy number increased significantly: 50%, 100% and 40% respectively at 125 mM excess phosphate. This was, however, accompanied by 26% decrease in specific growth rate and 30% in cellular growth yield coefficient.
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology