Abstract
We have used the polymerase chain reaction (PCR) with primers against the S5 and S6 regions of voltage-gated K+ channels to identify 8 different specific amplification products using poly(A)+ RNA isolated from islets of Langerhans from obese hyperglycémic (ob/ob) mice and from the two insulin-producing cell lines HIT T15 and RINmSF. Sequence analysis suggests that they derive from mRNAs coding for a family of voltage-gated K+ channels; 5 of these have been recently identified in mammalian brain and 3 are novel. These hybridize in classes to different mRNAs which distribute differently to a number of tissues and cell lines including insulin-producing cells.
| Original language | English |
|---|---|
| Pages (from-to) | 121-126 |
| Number of pages | 6 |
| Journal | FEBS Letters |
| Volume | 263 |
| Issue number | 1 |
| DOIs | |
| State | Published - Apr 9 1990 |
| Externally published | Yes |
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Keywords
- Insulin-producing cell
- Northern blot analysis
- Polymerase chain reaction
- Potassium channel
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Expression of voltage-gated K+ channels in insulin-producing cells. Analysis by polymerase chain reaction. / Betsholtz, Christer; Baumann, Arnd; Kenna, Susan; Ashcroft, Frances M.; Ashcroft, Stephen J H; Berggren, Per Olof; Grupe, Andrew; Pongs, Olaf; Rorsman, Patrik; Sandblom, John; Welsh, Michael.
In: FEBS Letters, Vol. 263, No. 1, 09.04.1990, p. 121-126.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of voltage-gated K+ channels in insulin-producing cells. Analysis by polymerase chain reaction
AU - Betsholtz, Christer
AU - Baumann, Arnd
AU - Kenna, Susan
AU - Ashcroft, Frances M.
AU - Ashcroft, Stephen J H
AU - Berggren, Per Olof
AU - Grupe, Andrew
AU - Pongs, Olaf
AU - Rorsman, Patrik
AU - Sandblom, John
AU - Welsh, Michael
PY - 1990/4/9
Y1 - 1990/4/9
N2 - We have used the polymerase chain reaction (PCR) with primers against the S5 and S6 regions of voltage-gated K+ channels to identify 8 different specific amplification products using poly(A)+ RNA isolated from islets of Langerhans from obese hyperglycémic (ob/ob) mice and from the two insulin-producing cell lines HIT T15 and RINmSF. Sequence analysis suggests that they derive from mRNAs coding for a family of voltage-gated K+ channels; 5 of these have been recently identified in mammalian brain and 3 are novel. These hybridize in classes to different mRNAs which distribute differently to a number of tissues and cell lines including insulin-producing cells.
AB - We have used the polymerase chain reaction (PCR) with primers against the S5 and S6 regions of voltage-gated K+ channels to identify 8 different specific amplification products using poly(A)+ RNA isolated from islets of Langerhans from obese hyperglycémic (ob/ob) mice and from the two insulin-producing cell lines HIT T15 and RINmSF. Sequence analysis suggests that they derive from mRNAs coding for a family of voltage-gated K+ channels; 5 of these have been recently identified in mammalian brain and 3 are novel. These hybridize in classes to different mRNAs which distribute differently to a number of tissues and cell lines including insulin-producing cells.
KW - Insulin-producing cell
KW - Northern blot analysis
KW - Polymerase chain reaction
KW - Potassium channel
UR - http://www.scopus.com/inward/record.url?scp=0025250335&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025250335&partnerID=8YFLogxK
U2 - 10.1016/0014-5793(90)80719-Y
DO - 10.1016/0014-5793(90)80719-Y
M3 - Article
C2 - 1691985
AN - SCOPUS:0025250335
VL - 263
SP - 121
EP - 126
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 1
ER -