Abstract
We have used the polymerase chain reaction (PCR) with primers against the S5 and S6 regions of voltage-gated K+ channels to identify 8 different specific amplification products using poly(A)+ RNA isolated from islets of Langerhans from obese hyperglycémic (ob/ob) mice and from the two insulin-producing cell lines HIT T15 and RINmSF. Sequence analysis suggests that they derive from mRNAs coding for a family of voltage-gated K+ channels; 5 of these have been recently identified in mammalian brain and 3 are novel. These hybridize in classes to different mRNAs which distribute differently to a number of tissues and cell lines including insulin-producing cells.
Original language | English (US) |
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Pages (from-to) | 121-126 |
Number of pages | 6 |
Journal | FEBS letters |
Volume | 263 |
Issue number | 1 |
DOIs | |
State | Published - Apr 9 1990 |
Externally published | Yes |
Keywords
- Insulin-producing cell
- Northern blot analysis
- Polymerase chain reaction
- Potassium channel
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology