@article{ef716634f2244904a6a22cee35fb15dd,
title = "Expression of voltage-gated K+ channels in insulin-producing cells. Analysis by polymerase chain reaction",
abstract = "We have used the polymerase chain reaction (PCR) with primers against the S5 and S6 regions of voltage-gated K+ channels to identify 8 different specific amplification products using poly(A)+ RNA isolated from islets of Langerhans from obese hyperglyc{\'e}mic (ob/ob) mice and from the two insulin-producing cell lines HIT T15 and RINmSF. Sequence analysis suggests that they derive from mRNAs coding for a family of voltage-gated K+ channels; 5 of these have been recently identified in mammalian brain and 3 are novel. These hybridize in classes to different mRNAs which distribute differently to a number of tissues and cell lines including insulin-producing cells.",
keywords = "Insulin-producing cell, Northern blot analysis, Polymerase chain reaction, Potassium channel",
author = "Christer Betsholtz and Arnd Baumann and Susan Kenna and Ashcroft, {Frances M.} and Ashcroft, {Stephen J.H.} and Berggren, {Per Olof} and Andrew Grupe and Olaf Pongs and Patrik Rorsman and John Sandblom and Michael Welsh",
note = "Funding Information: AcknowledgemenTthse: t echnicaal ssistancoef VivekaS venssona nd S&run Svanholmi s gratefullya cknowledgedT. he work was supported by the Juvenile DiabetesF oundation Int., The Swedish Medical Research Council (12x-8273,1 2x-109, 19x-00034a nd 4x-08641)T, he Bank of Sweden TercentenaryF oundation, The Swedish Diabetes Association, The Hoechst Foundation, The Swedish Cancer ResearchF und, The Medical ResearchC ouncil (UK), the British DiabeticA ssociation,E .P. AbrahamF und and the EuropeanM olecularB iologyO rganizationF. .M.A. is a Royal Society 1983 UniversitRye searchF ellow.",
year = "1990",
month = apr,
day = "9",
doi = "10.1016/0014-5793(90)80719-Y",
language = "English (US)",
volume = "263",
pages = "121--126",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "1",
}