Expression of voltage-gated K+ channels in insulin-producing cells. Analysis by polymerase chain reaction

Christer Betsholtz, Arnd Baumann, Susan Kenna, Frances M. Ashcroft, Stephen J.H. Ashcroft, Per Olof Berggren, Andrew Grupe, Olaf Pongs, Patrik Rorsman, John Sandblom, Michael Welsh

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

We have used the polymerase chain reaction (PCR) with primers against the S5 and S6 regions of voltage-gated K+ channels to identify 8 different specific amplification products using poly(A)+ RNA isolated from islets of Langerhans from obese hyperglycémic (ob/ob) mice and from the two insulin-producing cell lines HIT T15 and RINmSF. Sequence analysis suggests that they derive from mRNAs coding for a family of voltage-gated K+ channels; 5 of these have been recently identified in mammalian brain and 3 are novel. These hybridize in classes to different mRNAs which distribute differently to a number of tissues and cell lines including insulin-producing cells.

Original languageEnglish (US)
Pages (from-to)121-126
Number of pages6
JournalFEBS letters
Volume263
Issue number1
DOIs
StatePublished - Apr 9 1990

Keywords

  • Insulin-producing cell
  • Northern blot analysis
  • Polymerase chain reaction
  • Potassium channel

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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