Expression of voltage-gated K+ channels in insulin-producing cells. Analysis by polymerase chain reaction

Christer Betsholtz; Arnd Baumann; Susan Kenna; Frances M. Ashcroft; Stephen J.H. Ashcroft; Per Olof Berggren; Andrew Grupe; Olaf Pongs; Patrik Rorsman; John Sandblom; Michael Welsh

doi:10.1016/0014-5793(90)80719-Y

Expression of voltage-gated K⁺ channels in insulin-producing cells. Analysis by polymerase chain reaction

Christer Betsholtz, Arnd Baumann, Susan Kenna, Frances M. Ashcroft, Stephen J.H. Ashcroft, Per Olof Berggren, Andrew Grupe, Olaf Pongs, Patrik Rorsman, John Sandblom, Michael Welsh

Surgery

Research output: Contribution to journal › Article

31 Scopus citations

Abstract

We have used the polymerase chain reaction (PCR) with primers against the S5 and S6 regions of voltage-gated K⁺ channels to identify 8 different specific amplification products using poly(A)⁺ RNA isolated from islets of Langerhans from obese hyperglycémic (ob/ob) mice and from the two insulin-producing cell lines HIT T15 and RINmSF. Sequence analysis suggests that they derive from mRNAs coding for a family of voltage-gated K⁺ channels; 5 of these have been recently identified in mammalian brain and 3 are novel. These hybridize in classes to different mRNAs which distribute differently to a number of tissues and cell lines including insulin-producing cells.

Original language	English (US)
Pages (from-to)	121-126
Number of pages	6
Journal	FEBS letters
Volume	263
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(90)80719-Y
State	Published - Apr 9 1990

Keywords

Insulin-producing cell
Northern blot analysis
Polymerase chain reaction
Potassium channel

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

Access to Document

10.1016/0014-5793(90)80719-Y

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Expression of voltage-gated K⁺ channels in insulin-producing cells. Analysis by polymerase chain reaction. / Betsholtz, Christer; Baumann, Arnd; Kenna, Susan; Ashcroft, Frances M.; Ashcroft, Stephen J.H.; Berggren, Per Olof; Grupe, Andrew; Pongs, Olaf; Rorsman, Patrik; Sandblom, John; Welsh, Michael.

In: FEBS letters, Vol. 263, No. 1, 09.04.1990, p. 121-126.

Research output: Contribution to journal › Article

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AU - Ashcroft, Stephen J.H.

AU - Berggren, Per Olof

AU - Grupe, Andrew

AU - Pongs, Olaf

AU - Rorsman, Patrik

AU - Sandblom, John

AU - Welsh, Michael

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