Expression of voltage-gated K+ channels in insulin-producing cells. Analysis by polymerase chain reaction

Christer Betsholtz, Arnd Baumann, Susan Kenna, Frances M. Ashcroft, Stephen J H Ashcroft, Per Olof Berggren, Andrew Grupe, Olaf Pongs, Patrik Rorsman, John Sandblom, Michael Welsh

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

We have used the polymerase chain reaction (PCR) with primers against the S5 and S6 regions of voltage-gated K+ channels to identify 8 different specific amplification products using poly(A)+ RNA isolated from islets of Langerhans from obese hyperglycémic (ob/ob) mice and from the two insulin-producing cell lines HIT T15 and RINmSF. Sequence analysis suggests that they derive from mRNAs coding for a family of voltage-gated K+ channels; 5 of these have been recently identified in mammalian brain and 3 are novel. These hybridize in classes to different mRNAs which distribute differently to a number of tissues and cell lines including insulin-producing cells.

Original languageEnglish
Pages (from-to)121-126
Number of pages6
JournalFEBS Letters
Volume263
Issue number1
DOIs
StatePublished - Apr 9 1990
Externally publishedYes

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Voltage-Gated Potassium Channels
Polymerase chain reaction
Insulin
Polymerase Chain Reaction
Messenger RNA
Cells
S 6
Cell Line
Islets of Langerhans
Amplification
Sequence Analysis
Brain
Tissue

Keywords

  • Insulin-producing cell
  • Northern blot analysis
  • Polymerase chain reaction
  • Potassium channel

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Betsholtz, C., Baumann, A., Kenna, S., Ashcroft, F. M., Ashcroft, S. J. H., Berggren, P. O., ... Welsh, M. (1990). Expression of voltage-gated K+ channels in insulin-producing cells. Analysis by polymerase chain reaction. FEBS Letters, 263(1), 121-126. https://doi.org/10.1016/0014-5793(90)80719-Y

Expression of voltage-gated K+ channels in insulin-producing cells. Analysis by polymerase chain reaction. / Betsholtz, Christer; Baumann, Arnd; Kenna, Susan; Ashcroft, Frances M.; Ashcroft, Stephen J H; Berggren, Per Olof; Grupe, Andrew; Pongs, Olaf; Rorsman, Patrik; Sandblom, John; Welsh, Michael.

In: FEBS Letters, Vol. 263, No. 1, 09.04.1990, p. 121-126.

Research output: Contribution to journalArticle

Betsholtz, C, Baumann, A, Kenna, S, Ashcroft, FM, Ashcroft, SJH, Berggren, PO, Grupe, A, Pongs, O, Rorsman, P, Sandblom, J & Welsh, M 1990, 'Expression of voltage-gated K+ channels in insulin-producing cells. Analysis by polymerase chain reaction', FEBS Letters, vol. 263, no. 1, pp. 121-126. https://doi.org/10.1016/0014-5793(90)80719-Y
Betsholtz C, Baumann A, Kenna S, Ashcroft FM, Ashcroft SJH, Berggren PO et al. Expression of voltage-gated K+ channels in insulin-producing cells. Analysis by polymerase chain reaction. FEBS Letters. 1990 Apr 9;263(1):121-126. https://doi.org/10.1016/0014-5793(90)80719-Y
Betsholtz, Christer ; Baumann, Arnd ; Kenna, Susan ; Ashcroft, Frances M. ; Ashcroft, Stephen J H ; Berggren, Per Olof ; Grupe, Andrew ; Pongs, Olaf ; Rorsman, Patrik ; Sandblom, John ; Welsh, Michael. / Expression of voltage-gated K+ channels in insulin-producing cells. Analysis by polymerase chain reaction. In: FEBS Letters. 1990 ; Vol. 263, No. 1. pp. 121-126.
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