Expression of uncoupling protein 1 in mouse brown adipose tissue is thyroid hormone receptor-β isoform specific and required for adaptive thermogenesis

Miriam O. Ribeiro, Suzy D C Bianco, Masahiro Kaneshige, James J. Schultz, Sheue Yann Cheng, Antonio C. Bianco, Gregory A. Brent

Research output: Contribution to journalArticle

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Abstract

Cold-induced adaptive (or nonshivering) thermogenesis in small mammals is produced primarily in brown adipose tissue (BAT). BAT has been identified in humans and becomes more active after cold exposure. Heat production from BAT requires sympathetic nervous system stimulation, T3, and uncoupling protein 1 (UCP1) expression. Our previous studies with a thyroid hormone receptor-β (TRβ) isoform-selective agonist demonstrated that after TRβ stimulation alone, adaptive thermogenesis was markedly impaired, although UCP-1 expression in BAT was normal. We used mice with a dominant-negative TRβ PV mutation (frameshift mutation in resistance to thyroid hormone patient PV) to determine the role of TRβ in adaptive thermogenesisc and UCP1expression. Wild-type and PV mutant mice were made hypothyroid and replaced with T3 (7 ng/g·d) for 10 d to produce similar serum thyroid hormone concentration in the wild-type and mutant mice. The thermogenic response of interscapular BAT, as determined by heat production during iv infusions of norepinephrine, was reduced in PVβ heterozygous and homozygous mutant mice. The level of UCP1, the key thermogenic protein in BAT, was progressively reduced in PVβ+/- and PVβ-/- mutant mice. Brown adipocytes isolated from PV mutant mice had some reduction in cAMP and glycerol production in response to adrenergic stimulation. Defective adaptive thermogenesis in TRβ PV mutant mice is due to reduced UCP1 expression and reduced adrenergic responsiveness. TRβ mediates T3 regulation of UCP1 in BAT and is required for adaptive thermogenesis.

Original languageEnglish
Pages (from-to)432-440
Number of pages9
JournalEndocrinology
Volume151
Issue number1
DOIs
StatePublished - Jan 1 2010

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Thyroid Hormone Receptors
Brown Adipose Tissue
Thermogenesis
Protein Isoforms
Adrenergic Agents
Thyroid Hormone Resistance Syndrome
Brown Adipocytes
Frameshift Mutation
Sympathetic Nervous System
Uncoupling Protein 1
Thyroid Hormones
Glycerol
Mammals
Norepinephrine
Mutation
Serum

ASJC Scopus subject areas

  • Endocrinology

Cite this

Ribeiro, M. O., Bianco, S. D. C., Kaneshige, M., Schultz, J. J., Cheng, S. Y., Bianco, A. C., & Brent, G. A. (2010). Expression of uncoupling protein 1 in mouse brown adipose tissue is thyroid hormone receptor-β isoform specific and required for adaptive thermogenesis. Endocrinology, 151(1), 432-440. https://doi.org/10.1210/en.2009-0667

Expression of uncoupling protein 1 in mouse brown adipose tissue is thyroid hormone receptor-β isoform specific and required for adaptive thermogenesis. / Ribeiro, Miriam O.; Bianco, Suzy D C; Kaneshige, Masahiro; Schultz, James J.; Cheng, Sheue Yann; Bianco, Antonio C.; Brent, Gregory A.

In: Endocrinology, Vol. 151, No. 1, 01.01.2010, p. 432-440.

Research output: Contribution to journalArticle

Ribeiro, Miriam O. ; Bianco, Suzy D C ; Kaneshige, Masahiro ; Schultz, James J. ; Cheng, Sheue Yann ; Bianco, Antonio C. ; Brent, Gregory A. / Expression of uncoupling protein 1 in mouse brown adipose tissue is thyroid hormone receptor-β isoform specific and required for adaptive thermogenesis. In: Endocrinology. 2010 ; Vol. 151, No. 1. pp. 432-440.
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