Expression of the zinc-finger gene PLZF at rhombomere boundaries in the vertebrate hindbrain

M. Cook, A. Gould, N. Brand, J. Davies, P. Strutt, R. Shaknovich, J. Licht, S. Waxman, Z. Chen, S. Gluecksohn-Waelsch, R. Krumlauf, Arthur Z Zelent

Research output: Contribution to journalArticle

107 Citations (Scopus)

Abstract

To investigate the potential biological role(s) of the PLZF gene, discovered as a fusion with the RARA locus in a patient with acute promyelocytic leukemia harboring a t(11;17) chromosomal translocation, we have isolated its murine homologue (mPLZF) and studied its patterns of developmental expression. The levels of mPLZF mRNAs increased perinatally in the liver, heart, and kidney, but with the exception of the heart, they were either absent or very low in the adult tissues. In situ analysis of mPLZF expression in mouse embryos between 7.0 and 10.5 days of development revealed that mPLZF mRNAs and proteins were coexpressed in spatially restricted and temporally dynamic patterns in the central nervous system. In the hindbrain region, a segmental pattern of expression correlated with the development of the rhombomeres. From 9.0 days of development, starting first in rhombomeres 3 and 5, there was an ordered down-regulation of expression in the center of each rhombomere, so that 1 day later elevated levels of mPLZF mRNAs and proteins were restricted to cells surrounding the rhombomeric boundaries. The chicken homologue of the PLZF gene, which we have also cloned, demonstrated a similar segmental pattern of expression in the hindbrain. To date, PLZF represents the only example of a transcription factor with elevated expression at rhombomeric boundaries. The high degree of evolutionary conservation between the patterns of PLZF expression during mammalian and avian central nervous system development suggests that it has an important functional role in the regionalization of the vertebrate hindbrain, potentially regulating boundary cell interactions.

Original languageEnglish (US)
Pages (from-to)2249-2253
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume92
Issue number6
DOIs
StatePublished - Mar 14 1995
Externally publishedYes

Fingerprint

Rhombencephalon
Zinc Fingers
Vertebrates
Messenger RNA
Central Nervous System
Genes
Genetic Translocation
Acute Promyelocytic Leukemia
Cell Communication
Chickens
Proteins
Transcription Factors
Down-Regulation
Embryonic Structures
Kidney
Liver

Keywords

  • leukemia
  • liver
  • segmentation
  • transcription factor
  • translocation

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Expression of the zinc-finger gene PLZF at rhombomere boundaries in the vertebrate hindbrain. / Cook, M.; Gould, A.; Brand, N.; Davies, J.; Strutt, P.; Shaknovich, R.; Licht, J.; Waxman, S.; Chen, Z.; Gluecksohn-Waelsch, S.; Krumlauf, R.; Zelent, Arthur Z.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, No. 6, 14.03.1995, p. 2249-2253.

Research output: Contribution to journalArticle

Cook, M, Gould, A, Brand, N, Davies, J, Strutt, P, Shaknovich, R, Licht, J, Waxman, S, Chen, Z, Gluecksohn-Waelsch, S, Krumlauf, R & Zelent, AZ 1995, 'Expression of the zinc-finger gene PLZF at rhombomere boundaries in the vertebrate hindbrain', Proceedings of the National Academy of Sciences of the United States of America, vol. 92, no. 6, pp. 2249-2253. https://doi.org/10.1073/pnas.92.6.2249
Cook, M. ; Gould, A. ; Brand, N. ; Davies, J. ; Strutt, P. ; Shaknovich, R. ; Licht, J. ; Waxman, S. ; Chen, Z. ; Gluecksohn-Waelsch, S. ; Krumlauf, R. ; Zelent, Arthur Z. / Expression of the zinc-finger gene PLZF at rhombomere boundaries in the vertebrate hindbrain. In: Proceedings of the National Academy of Sciences of the United States of America. 1995 ; Vol. 92, No. 6. pp. 2249-2253.
@article{c981f1878fd246469577494fe9834253,
title = "Expression of the zinc-finger gene PLZF at rhombomere boundaries in the vertebrate hindbrain",
abstract = "To investigate the potential biological role(s) of the PLZF gene, discovered as a fusion with the RARA locus in a patient with acute promyelocytic leukemia harboring a t(11;17) chromosomal translocation, we have isolated its murine homologue (mPLZF) and studied its patterns of developmental expression. The levels of mPLZF mRNAs increased perinatally in the liver, heart, and kidney, but with the exception of the heart, they were either absent or very low in the adult tissues. In situ analysis of mPLZF expression in mouse embryos between 7.0 and 10.5 days of development revealed that mPLZF mRNAs and proteins were coexpressed in spatially restricted and temporally dynamic patterns in the central nervous system. In the hindbrain region, a segmental pattern of expression correlated with the development of the rhombomeres. From 9.0 days of development, starting first in rhombomeres 3 and 5, there was an ordered down-regulation of expression in the center of each rhombomere, so that 1 day later elevated levels of mPLZF mRNAs and proteins were restricted to cells surrounding the rhombomeric boundaries. The chicken homologue of the PLZF gene, which we have also cloned, demonstrated a similar segmental pattern of expression in the hindbrain. To date, PLZF represents the only example of a transcription factor with elevated expression at rhombomeric boundaries. The high degree of evolutionary conservation between the patterns of PLZF expression during mammalian and avian central nervous system development suggests that it has an important functional role in the regionalization of the vertebrate hindbrain, potentially regulating boundary cell interactions.",
keywords = "leukemia, liver, segmentation, transcription factor, translocation",
author = "M. Cook and A. Gould and N. Brand and J. Davies and P. Strutt and R. Shaknovich and J. Licht and S. Waxman and Z. Chen and S. Gluecksohn-Waelsch and R. Krumlauf and Zelent, {Arthur Z}",
year = "1995",
month = "3",
day = "14",
doi = "10.1073/pnas.92.6.2249",
language = "English (US)",
volume = "92",
pages = "2249--2253",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "6",

}

TY - JOUR

T1 - Expression of the zinc-finger gene PLZF at rhombomere boundaries in the vertebrate hindbrain

AU - Cook, M.

AU - Gould, A.

AU - Brand, N.

AU - Davies, J.

AU - Strutt, P.

AU - Shaknovich, R.

AU - Licht, J.

AU - Waxman, S.

AU - Chen, Z.

AU - Gluecksohn-Waelsch, S.

AU - Krumlauf, R.

AU - Zelent, Arthur Z

PY - 1995/3/14

Y1 - 1995/3/14

N2 - To investigate the potential biological role(s) of the PLZF gene, discovered as a fusion with the RARA locus in a patient with acute promyelocytic leukemia harboring a t(11;17) chromosomal translocation, we have isolated its murine homologue (mPLZF) and studied its patterns of developmental expression. The levels of mPLZF mRNAs increased perinatally in the liver, heart, and kidney, but with the exception of the heart, they were either absent or very low in the adult tissues. In situ analysis of mPLZF expression in mouse embryos between 7.0 and 10.5 days of development revealed that mPLZF mRNAs and proteins were coexpressed in spatially restricted and temporally dynamic patterns in the central nervous system. In the hindbrain region, a segmental pattern of expression correlated with the development of the rhombomeres. From 9.0 days of development, starting first in rhombomeres 3 and 5, there was an ordered down-regulation of expression in the center of each rhombomere, so that 1 day later elevated levels of mPLZF mRNAs and proteins were restricted to cells surrounding the rhombomeric boundaries. The chicken homologue of the PLZF gene, which we have also cloned, demonstrated a similar segmental pattern of expression in the hindbrain. To date, PLZF represents the only example of a transcription factor with elevated expression at rhombomeric boundaries. The high degree of evolutionary conservation between the patterns of PLZF expression during mammalian and avian central nervous system development suggests that it has an important functional role in the regionalization of the vertebrate hindbrain, potentially regulating boundary cell interactions.

AB - To investigate the potential biological role(s) of the PLZF gene, discovered as a fusion with the RARA locus in a patient with acute promyelocytic leukemia harboring a t(11;17) chromosomal translocation, we have isolated its murine homologue (mPLZF) and studied its patterns of developmental expression. The levels of mPLZF mRNAs increased perinatally in the liver, heart, and kidney, but with the exception of the heart, they were either absent or very low in the adult tissues. In situ analysis of mPLZF expression in mouse embryos between 7.0 and 10.5 days of development revealed that mPLZF mRNAs and proteins were coexpressed in spatially restricted and temporally dynamic patterns in the central nervous system. In the hindbrain region, a segmental pattern of expression correlated with the development of the rhombomeres. From 9.0 days of development, starting first in rhombomeres 3 and 5, there was an ordered down-regulation of expression in the center of each rhombomere, so that 1 day later elevated levels of mPLZF mRNAs and proteins were restricted to cells surrounding the rhombomeric boundaries. The chicken homologue of the PLZF gene, which we have also cloned, demonstrated a similar segmental pattern of expression in the hindbrain. To date, PLZF represents the only example of a transcription factor with elevated expression at rhombomeric boundaries. The high degree of evolutionary conservation between the patterns of PLZF expression during mammalian and avian central nervous system development suggests that it has an important functional role in the regionalization of the vertebrate hindbrain, potentially regulating boundary cell interactions.

KW - leukemia

KW - liver

KW - segmentation

KW - transcription factor

KW - translocation

UR - http://www.scopus.com/inward/record.url?scp=0028956073&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028956073&partnerID=8YFLogxK

U2 - 10.1073/pnas.92.6.2249

DO - 10.1073/pnas.92.6.2249

M3 - Article

C2 - 7892256

AN - SCOPUS:0028956073

VL - 92

SP - 2249

EP - 2253

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 6

ER -