Expression of the protein zero myelin gene in axon-related Schwann cells is linked to basal lamina formation

Cristina Fernandez-Valle, Nevis L. Fregien, Patrick Wood, Mary B Bunge

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

A Schwann cell has the potential to differentiate into either a myelinating or ensheathing cell depending upon signals received from the axon that it contacts. Studies focusing on the pathway leading to myelination demonstrated that Schwann cells must form a basal lamina in order to myelinate an axon. In this report, we describe studies that indicate that initiation of basal lamina synthesis is required for Schwann cells to distinguish between myelination-inducing axons and axons that do not induce myelination, and to respond by undergoing the appropriate genetic and cellular changes. We have used high resolution in situ hybridization, immunocytochemistry and electron microscopy to examine changes in gene expression and morphology of Schwann cells differentiating into myelin-forming cells in vitro. These experiments were carried out in dorsal root ganglion neuron/Schwann cell co-cultures maintained in either serum-free, serum-only or serum-plus-ascorbate-containing medium. We have made four novel observations that contribute significantly to our understanding of how basal lamina and myelination are linked. (1) The addition of ascorbate (in the presence of serum), which promotes basal lamina production, appears to induce expression of the protein zero gene encoding the major structural protein of myelin. Moreover, expression of protein zero mRNA and protein, and its insertion into myelin membranes, occurs only in the subset of Schwann cells contacting myelination-inducing axons. Schwann cells in contact with axons that do not induce myelination, or Schwann cells that have not established a unitary relationship with an axon, do not express protein zero mRNA although they produce basal lamina components. (2) In serum-free conditions, a majority of Schwann cells express protein zero mRNA and protein, but this change in gene expression is not associated with basal lamina formation or with elongation of the Schwann cell along the axon and elaboration of myelin. (3) In the presence of serum (and the absence of ascorbate), Schwann cells again fail to form basal lamina or elongate but no longer express protein zero mRNA or protein. (4) Myelin-associated glycoprotein and galactocerebroside, two additional myelin-specific components, can be expressed by Schwann cells under any of the three culture conditions. Therefore, we have demonstrated that axonal induction of protein zero gene expression in Schwann cells is subject to regulation by both serum- and ascorbate-dependent pathways and that not all myelin-specific proteins are regulated in the same manner. Only when Schwann cells contact axons and initiate basal lamina synthesis is expression of myelin-specific genes restricted to the subset of Schwann cells contacting myelination-inducing axons and coupled to cellular differentiation. In the absence of basal lamina formation, Schwann cells in contact with axons seem to express myelin-specific proteins spuriously without undergoing further differentiation. In sum, these findings suggest that basal lamina serves to simultaneously induce myelin gene expression and cell shape changes in those Schwann cells associated with axons destined for myelination. Basal lamina also suppresses the expression of myelin genes in these SCs in contact with axons that do not induce myelination.

Original languageEnglish
Pages (from-to)867-880
Number of pages14
JournalDevelopment
Volume119
Issue number3
StatePublished - Nov 1 1993

Fingerprint

Myelin P0 Protein
Schwann Cells
Basement Membrane
Axons
Genes
Myelin Sheath
Myelin Proteins
Proteins
Serum
Gene Expression
Messenger RNA
Myelin-Associated Glycoprotein

Keywords

  • Ascorbate
  • Basal lamina
  • In situ hybridization
  • Myelin-specific gene expression
  • Myelination
  • Protein zero
  • Schwann cell

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy

Cite this

Expression of the protein zero myelin gene in axon-related Schwann cells is linked to basal lamina formation. / Fernandez-Valle, Cristina; Fregien, Nevis L.; Wood, Patrick; Bunge, Mary B.

In: Development, Vol. 119, No. 3, 01.11.1993, p. 867-880.

Research output: Contribution to journalArticle

Fernandez-Valle, Cristina ; Fregien, Nevis L. ; Wood, Patrick ; Bunge, Mary B. / Expression of the protein zero myelin gene in axon-related Schwann cells is linked to basal lamina formation. In: Development. 1993 ; Vol. 119, No. 3. pp. 867-880.
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T1 - Expression of the protein zero myelin gene in axon-related Schwann cells is linked to basal lamina formation

AU - Fernandez-Valle, Cristina

AU - Fregien, Nevis L.

AU - Wood, Patrick

AU - Bunge, Mary B

PY - 1993/11/1

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N2 - A Schwann cell has the potential to differentiate into either a myelinating or ensheathing cell depending upon signals received from the axon that it contacts. Studies focusing on the pathway leading to myelination demonstrated that Schwann cells must form a basal lamina in order to myelinate an axon. In this report, we describe studies that indicate that initiation of basal lamina synthesis is required for Schwann cells to distinguish between myelination-inducing axons and axons that do not induce myelination, and to respond by undergoing the appropriate genetic and cellular changes. We have used high resolution in situ hybridization, immunocytochemistry and electron microscopy to examine changes in gene expression and morphology of Schwann cells differentiating into myelin-forming cells in vitro. These experiments were carried out in dorsal root ganglion neuron/Schwann cell co-cultures maintained in either serum-free, serum-only or serum-plus-ascorbate-containing medium. We have made four novel observations that contribute significantly to our understanding of how basal lamina and myelination are linked. (1) The addition of ascorbate (in the presence of serum), which promotes basal lamina production, appears to induce expression of the protein zero gene encoding the major structural protein of myelin. Moreover, expression of protein zero mRNA and protein, and its insertion into myelin membranes, occurs only in the subset of Schwann cells contacting myelination-inducing axons. Schwann cells in contact with axons that do not induce myelination, or Schwann cells that have not established a unitary relationship with an axon, do not express protein zero mRNA although they produce basal lamina components. (2) In serum-free conditions, a majority of Schwann cells express protein zero mRNA and protein, but this change in gene expression is not associated with basal lamina formation or with elongation of the Schwann cell along the axon and elaboration of myelin. (3) In the presence of serum (and the absence of ascorbate), Schwann cells again fail to form basal lamina or elongate but no longer express protein zero mRNA or protein. (4) Myelin-associated glycoprotein and galactocerebroside, two additional myelin-specific components, can be expressed by Schwann cells under any of the three culture conditions. Therefore, we have demonstrated that axonal induction of protein zero gene expression in Schwann cells is subject to regulation by both serum- and ascorbate-dependent pathways and that not all myelin-specific proteins are regulated in the same manner. Only when Schwann cells contact axons and initiate basal lamina synthesis is expression of myelin-specific genes restricted to the subset of Schwann cells contacting myelination-inducing axons and coupled to cellular differentiation. In the absence of basal lamina formation, Schwann cells in contact with axons seem to express myelin-specific proteins spuriously without undergoing further differentiation. In sum, these findings suggest that basal lamina serves to simultaneously induce myelin gene expression and cell shape changes in those Schwann cells associated with axons destined for myelination. Basal lamina also suppresses the expression of myelin genes in these SCs in contact with axons that do not induce myelination.

AB - A Schwann cell has the potential to differentiate into either a myelinating or ensheathing cell depending upon signals received from the axon that it contacts. Studies focusing on the pathway leading to myelination demonstrated that Schwann cells must form a basal lamina in order to myelinate an axon. In this report, we describe studies that indicate that initiation of basal lamina synthesis is required for Schwann cells to distinguish between myelination-inducing axons and axons that do not induce myelination, and to respond by undergoing the appropriate genetic and cellular changes. We have used high resolution in situ hybridization, immunocytochemistry and electron microscopy to examine changes in gene expression and morphology of Schwann cells differentiating into myelin-forming cells in vitro. These experiments were carried out in dorsal root ganglion neuron/Schwann cell co-cultures maintained in either serum-free, serum-only or serum-plus-ascorbate-containing medium. We have made four novel observations that contribute significantly to our understanding of how basal lamina and myelination are linked. (1) The addition of ascorbate (in the presence of serum), which promotes basal lamina production, appears to induce expression of the protein zero gene encoding the major structural protein of myelin. Moreover, expression of protein zero mRNA and protein, and its insertion into myelin membranes, occurs only in the subset of Schwann cells contacting myelination-inducing axons. Schwann cells in contact with axons that do not induce myelination, or Schwann cells that have not established a unitary relationship with an axon, do not express protein zero mRNA although they produce basal lamina components. (2) In serum-free conditions, a majority of Schwann cells express protein zero mRNA and protein, but this change in gene expression is not associated with basal lamina formation or with elongation of the Schwann cell along the axon and elaboration of myelin. (3) In the presence of serum (and the absence of ascorbate), Schwann cells again fail to form basal lamina or elongate but no longer express protein zero mRNA or protein. (4) Myelin-associated glycoprotein and galactocerebroside, two additional myelin-specific components, can be expressed by Schwann cells under any of the three culture conditions. Therefore, we have demonstrated that axonal induction of protein zero gene expression in Schwann cells is subject to regulation by both serum- and ascorbate-dependent pathways and that not all myelin-specific proteins are regulated in the same manner. Only when Schwann cells contact axons and initiate basal lamina synthesis is expression of myelin-specific genes restricted to the subset of Schwann cells contacting myelination-inducing axons and coupled to cellular differentiation. In the absence of basal lamina formation, Schwann cells in contact with axons seem to express myelin-specific proteins spuriously without undergoing further differentiation. In sum, these findings suggest that basal lamina serves to simultaneously induce myelin gene expression and cell shape changes in those Schwann cells associated with axons destined for myelination. Basal lamina also suppresses the expression of myelin genes in these SCs in contact with axons that do not induce myelination.

KW - Ascorbate

KW - Basal lamina

KW - In situ hybridization

KW - Myelin-specific gene expression

KW - Myelination

KW - Protein zero

KW - Schwann cell

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