Expression of the N-type Ca2+ channels following nerve injury

D. Čížková, S. Jergová, M. Čížek, J. Maršala

Research output: Contribution to journalArticlepeer-review


Recent experiments have shown that Ca2+ channel blockers are effective in modulating pain behavior in acute and chronic pain models. In the present study we have studied alterations of the voltage-dependent calcium channels, the N-type in the spinal cord of rats, that were processed to a unilateral sciatic nerve ligation and allowed to survival one week. Thereafter, we have examined the onset of the N-type Ca2+ channel appearance in the corresponding spinal cord segments (L4-L6). Immunocytochemical analysis of the spinal cord in control rats showed a smooth diffuse N-type Ca2+ immunoreactivity in the superficial laminae I-II, as well as in ventral motoneurons. However, in the nerve-ligated animals an intense dot-like immunoreactivity in the ipsilateral dorsal horn was observed from 3-7 days after nerve ligation.. The most prominent N-type Ca2+ channel immunoreactivity was found in the outer as well as in the inner part of lamina II. These changes were typically seen in the L5-L4 spinal cord segments. The L3 and L6 segment showed only light increase of the N-type Ca2+ channel immunoreactivity in the laminae I-II.

Original languageEnglish (US)
Pages (from-to)16-17
Number of pages2
Issue numberSUPPL. 2
StatePublished - 2001
Externally publishedYes

ASJC Scopus subject areas

  • Psychiatry and Mental health


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